Development of Disease and Virus Recovery in Transgenic Mice Containing HIV Proviral DNA

Transgenic mice containing intact copies of the human immunodeficiency virus (HIV) proviral DNA were constructed. Founder animals were not viremic for HIV and remained healthy during a 9-month observation period. After being mated with nontransgenic animals, one founder mouse (No. 13) gave rise to F...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1988-12, Vol.242 (4886), p.1665-1670
Main Authors: Leonard, John M., Abramczuk, Jan W., Pezen, David S., Rutledge, Rosamond, Belcher, J. Harry, Hakim, Frances, Shearer, Gene, Lamperth, Lajos, Travis, William, Fredrickson, Torgny, Notkins, Abner L., Martin, Malcolm A.
Format: Article
Language:English
Subjects:
Acquired immune deficiency syndrome
Acquired Immunodeficiency Syndrome - immunology
Acquired Immunodeficiency Syndrome - microbiology
Acquired Immunodeficiency Syndrome - pathology
AIDS
AIDS (Disease)
AIDS/HIV
Animals
Biotechnology
Disease Models, Animal
DNA
DNA Probes
DNA, Viral - analysis
Epidermis - pathology
Genetic aspects
Genetic transformation
Genetics
HIV
HIV (Viruses)
HIV - genetics
HIV - immunology
HIV - isolation & purification
HIV Antibodies - analysis
House mouse
Human immunodeficiency virus
Lung - pathology
Lymph nodes
Lymph Nodes - microbiology
Lymph Nodes - pathology
Lymphocytes
Medical research
Mice
Mice as laboratory animals
Mice, Transgenic
Nucleic Acid Hybridization
Rodents
Skin
Skin - microbiology
Skin - pathology
Spleen
Spleen - microbiology
Spleen - pathology
Transgenic animals
Viruses
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Summary:Transgenic mice containing intact copies of the human immunodeficiency virus (HIV) proviral DNA were constructed. Founder animals were not viremic for HIV and remained healthy during a 9-month observation period. After being mated with nontransgenic animals, one founder mouse (No. 13) gave rise to F$_{1}$ progeny that developed a disease syndrome characterized by marked epidermal hyperplasia, lymphadenopathy, splenomegaly, pulmonary lymphoid infiltrates, growth retardation, and death by day 25 of life. Infectious HIV, indistinguishable from parental virus by immunoblot analysis, was recovered from the spleen, lymph nodes, and skin of five of five affected animals.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3201255