Molecular Cloning of Two Types of GAP Complementary DNA from Human Placenta

The ras p21 GTPase-activating protein (GAP) was purified from human placental tissue. Internal amino acid sequence was obtained from this 120,000-dalton protein and, by means of this sequence, two types of complementary DNA clones were isolated and characterized. One type encoded GAP with a predicte...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1988-12, Vol.242 (4886), p.1697-1700
Main Authors: Trahey, Meg, Wong, Gail, Halenbeck, Robert, Rubinfeld, Bonnee, Martin, George A., Ladner, Martha, Long, Christopher M., Crosier, Walter J., Watt, Ken, Koths, Kirston, McCormick, Frank
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acid sequencing
Amino acids
Analysis
Base Sequence
Biological and medical sciences
Brain Chemistry
Cloning
Cloning, Molecular
Codons
Complementary DNA
DNA
DNA - genetics
DNA - isolation & purification
Female
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genetics
Good agricultural practices
GTPase-Activating Proteins
Humans
Leukocytes - analysis
Liver - analysis
Lung - analysis
Medical research
Messenger RNA
Molecular and cellular biology
Molecular cloning
Molecular genetics
Molecular Sequence Data
Molecular Weight
Nucleic Acid Hybridization
Oligonucleotide Probes
Oligonucleotides
Placenta
Placenta - analysis
Pregnancy
Proteins
Proteins - genetics
Proteins - isolation & purification
ras GTPase-Activating Proteins
RNA, Messenger - analysis
RNA, Messenger - genetics
Sequence Homology, Nucleic Acid
Transcription. Transcription factor. Splicing. Rna processing
Ungulates
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Summary:The ras p21 GTPase-activating protein (GAP) was purified from human placental tissue. Internal amino acid sequence was obtained from this 120,000-dalton protein and, by means of this sequence, two types of complementary DNA clones were isolated and characterized. One type encoded GAP with a predicted molecular mass of 116,000 daltons and 96% identity with bovine GAP. The messenger RNA of this GAP was detected in human lung, brain, liver, leukocytes, and placenta. The second type appeared to be generated by a differential splicing mechanism and encoded a novel form of GAP with a predicted molecular mass of 100,400 daltons. This protein lacks the hydrophobic amino terminus characteristic of the larger species, but retains GAP activity. The messenger RNA of this type was abundantly expressed in placenta and in several human cell lines, but not in adult tissues.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3201259