Expression and biochemical analyses of the recombinant potato virus X 25K movement protein

• Published in: FEBS letters 1996-11, Vol.397 (1), p.75-78
• Main Authors: Kalinina, N.O., Fedorkin, O.N., Samuilova, O.V., Maiss, E., Korpela, T., Morozov, S.Yu, Atabekov, J.G.
• Format: Article
• Language: English
• Subjects: Acid Anhydride Hydrolases - metabolism; Adenosine Triphosphatases - metabolism; Amino Acid Sequence; ATPase; Escherichia coli - genetics; Molecular Sequence Data; Molecular Weight; Movement protein; Nucleoside-Triphosphatase; Plant RNA virus; Plant Viral Movement Proteins; potato virus X; Potexvirus; Recombinant Proteins - chemistry; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; RNA - metabolism; RNA binding; RNA Helicases; RNA Nucleotidyltransferases - metabolism; RNA, Viral - pharmacology; RNA-Binding Proteins - metabolism; Viral Proteins - chemistry; Viral Proteins - genetics; Viral Proteins - isolation & purification; Viral Proteins - metabolism
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(96)01138-6

The 25K movement protein (MP) of potato virus X (PVX) is encoded by the 5′-proximal gene of three overlapping MP genes forming a ‘triple gene block’. The PVX 25K MP (putative NTPase-helicase) has been synthesized in Escherichia coli as a recombinant containing a six-histidine tag at the amino terminus. The His-tagged 25K protein was purified in a one-column Ni-chelate affinity chromatography procedure. In the absence of any other viral factors, this protein had obvious Mg 2+-dependent ATPase activity, which was stimulated slightly (1.7–1.9-fold) by various polynucleotides. Like other viral proteins possessing ATPase-helicase motifs and many plant viral movement proteins, the PVX 25K MP was able to bind nucleic acids in vitro. The RNA binding activity of the 25K MP was pronounced only at very low salt concentrations and was independent of its ATPase activity.