The presence of Müllerian inhibiting substance in human seminal plasma

Müllerian inhibiting substance (MIS), produced by testicular Sertoli cells, is present in adult male serum. The first aim of this study was to determine if MIS is present in seminal plasma. Using an enzyme-linked immunosorbent assay (ELISA), we measured MIS concentrations in seminal plasma from 23 d...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1996-10, Vol.11 (10), p.2165-2169
Main Authors: FALLAT, M. E, SIOW, Y, BELKER, A. M, BOYD, J. K, YOFFE, S, MACLAUGHLIN, D. T
Format: Article
Language:English
Subjects:
Adult
Anti-Mullerian Hormone
Biological and medical sciences
Centrifugation
Cryopreservation
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Glycoproteins
Growth Inhibitors - metabolism
Hormone metabolism and regulation
Humans
Infertility, Male - metabolism
Infertility, Male - physiopathology
Male
Mammalian male genital system
Recombinant Proteins
Reference Values
Semen - metabolism
Sperm Motility
Testicular Hormones - metabolism
Vertebrates: reproduction
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Summary:Müllerian inhibiting substance (MIS), produced by testicular Sertoli cells, is present in adult male serum. The first aim of this study was to determine if MIS is present in seminal plasma. Using an enzyme-linked immunosorbent assay (ELISA), we measured MIS concentrations in seminal plasma from 23 donors exhibiting normal (WHO criteria) sperm qualities, and 169 patients with subnormal sperm parameters. The second aim of this study was to examine a potential relationship between MIS and sperm motility. MIS concentrations in seminal plasma ranged from 0.5 to 3.6 ng/ml in donors and from 0.5 to 17.8 ng/ml in patients. Motility index (MI, mean +/- SEM) for all patient samples was lower compared with donors (113.3 +/- 3.2 and 198.3 +/- 13.5, P < 0.00001), while mean MIS concentration +/- SEM) was higher (4.2 +/- 0.3 and 1.4 +/- 0.2, P < 0.0003). When the patients were stratified into Groups I (motility < 50%, n = 42) and II (motility > 50%, n = 127), the MI (mean +/- SEM) values were 62.3 +/- 3.8 and 130.2 +/- 2.7 respectively (P < 0.0001 for both compared with donors) and mean MIS concentrations (+/- SEM) were 5.4 +/- 0.6 and 3.9 +/- 0.3, respectively (P < 0.0001 and P < 0.001 compared with donors). The inverse relationship between MIS concentration in seminal plasma and motility index suggests that MIS may have a function in modulating motility.
ISSN:0268-1161
1460-2350