Analysis of the oocyte activating capacity and chromosomal complement of round-headed human spermatozoa by their injection into mouse oocytes

Intracytoplasmic sperm injection (ICSI) in the human is a very effective procedure which allows the fertilization of the majority of oocytes even in cases of extreme oligoasthenoteratozoospermia. Round-headed acrosomeless human spermatozoa, however, form an exception to this rule, because in about h...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1996-10, Vol.11 (10), p.2170-2175
Main Authors: RYBOUCHKIN, A, DOZORTSEV, D, PELINCK, M. J, DE SUTTER, P, DHONT, M
Format: Article
Language:English
Subjects:
Adult
Animals
Biological and medical sciences
Birth control
Centromere - ultrastructure
Chromosome Aberrations
Chromosome Disorders
Chromosomes
Ethanol - pharmacology
Female
Gynecology. Andrology. Obstetrics
Humans
Karyotyping
Male
Medical sciences
Mice
Microinjections
Oocytes - drug effects
Oocytes - physiology
Sperm Head - ultrastructure
Sperm-Ovum Interactions
Spermatozoa - physiology
Spermatozoa - ultrastructure
Sterility. Assisted procreation
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Summary:Intracytoplasmic sperm injection (ICSI) in the human is a very effective procedure which allows the fertilization of the majority of oocytes even in cases of extreme oligoasthenoteratozoospermia. Round-headed acrosomeless human spermatozoa, however, form an exception to this rule, because in about half of the couples with globozoospermia all oocytes remain unfertilized after injection. The incapacity of the spermatozoon to activate the oocyte following injection of round-headed spermatozoa could be the underlying mechanism. To investigate this hypothesis, activation rates of mouse oocytes injected with spermatozoa from a patient with globozoospermia were compared with those obtained after injection with normal spermatozoa. Of mouse oocytes surviving the injection with donor spermatozoa, 95% underwent activation, compared to none of the 88 mouse oocytes surviving the injection with round-headed spermatozoa. After fixation, prematurely condensed sperm chromosomes were found in these oocytes. Parthenogenetic activation of mouse oocytes (8% ethanol at 40 min after injection) injected with round-headed spermatozoa led to the activation of 96% of oocytes. These oocytes developed normally to the first mitosis and were fixed for analysis of the sperm karyotypes. The incidence of chromosomal abnormalities of round-headed spermatozoa (6%) was similar to that in spermatozoa from a fertile donor (9%). These data provide further information on the basic defect in cases of globozoospermia and demonstrate that globozoospermia is not associated with sperm karyotype abnormalities.
ISSN:0268-1161
1460-2350