Rapid cytologic diagnosis of respiratory cryptosporidiosis in chickens

• Published in: Avian diseases 1988-10, Vol.32 (4), p.826-830
• Main Authors: Latimer, K.S, Goodwin, M.A, Davis, M.K
• Format: Article
• Language: English
• Subjects: Animals; CHICKENS; Chickens - parasitology; CITOLOGIA; Cryptosporidiosis; Cryptosporidiosis - diagnosis; CRYPTOSPORIDIUM; CYTOLOGIE; CYTOLOGY; DIAGNOSIS; DIAGNOSTIC; DIAGNOSTICO; ENFERMEDADES RESPIRATORIAS; Epithelial cells; Histology; MALADIE RESPIRATOIRE; Oocysts; Parasites; POLLO; POULET; Poultry Diseases - diagnosis; Poultry Diseases - parasitology; Prospective Studies; Research Notes; RESPIRATORY DISEASES; Staining and Labeling - methods; Sulfuric acids; Trachea - parasitology; Turkeys
• ISSN: 0005-2086; 1938-4351
• DOI: 10.2307/1591006

A method is presented for the rapid cytologic detection of cryptosporidial oocysts in Diff-Quik and modified Kinyoun acid-fast-stained tracheal imprints. On Diff-Quik-stained preparations, cryptosporidial oocysts were observed attached to the apical ends of respiratory epithelial cells or scattered throughout the imprint. These oocysts were round to oval, measured approximately 6 to 7 μm in diameter, and appeared faintly blue with fine pink granules. In modified Kinyoun acid-fast-stained tracheal imprints, the oocysts usually were pink to bright red against a blue background. Cytologic examination of tracheal imprints following Diff-Quik and modified Kinyoun acid-fast staining provides a rapid, reliable, and economical method for diagnosis of respiratory cryptosporidiosis in poultry. /// Se presenta un método para la detección citológica rápida de oocistos criptosporidiales por los métodos de tinción Diff-Quik y ácidoresistente modificada de Kinyoun en impresiones traqueales. En las preparaciones utilizando la tinción Diff-Quik, los oocistos criptosporidiales se observaron adheridos a las terminaciones apicales de las células epiteliales respiratorias o distribuidos a todo lo largo de la impresión. Estos oocistos tuvieron una forma redondeada a ovalada, midiendo aproximadamente entre 6 a 7 μm en diámetro y se observaron de un color azul opaco y con gránulos muy pequeños de color rosado. En impresiones traqueales utilizando la tinción ácidoresistente modificada de Kinyoun, los oocistos usualmente aparecieron de color rosado a rojo brillante contra un fondo azul. Los exámenes citológicos de impresiones traqueales después de las tinciones Diff-Quik y ácidoresistente modificada de Kinyoun constituyen un método rápido, confiable y económico para el diagnóstico de criptosporidiosis respiratoria en pollos.