Growth requirements, growth factor responsiveness, and growth factor secretion of three human embryonal carcinoma cell lines

The in vitro growth requirements of three human embryonal carcinoma cell lines (H 12.7, 2102 EP, 1428 A) were investigated. The basal medium DME/F12 supplemented with insulin, transferrin, and low-density and high-density lipoproteins was sufficient to support substantial multiplication of all three...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cancer research and clinical oncology 1988-12, Vol.114 (6), p.553-558
Main Authors: VERBEEK, W, BOKEMEYER, C, FALK, H, SCHMOLL, H.-J
Format: Article
Language:English
Subjects:
Animal cells
Biological and medical sciences
Cell cultures. Hybridization. Fusion
Cell Division
Culture Media
DNA - biosynthesis
Embryonal Carcinoma Stem Cells
ErbB Receptors - analysis
Fundamental and applied biological sciences. Psychology
Growth Substances - metabolism
Growth Substances - pharmacology
Humans
Molecular and cellular biology
Neoplastic Stem Cells - drug effects
Neoplastic Stem Cells - metabolism
Neoplastic Stem Cells - pathology
Tumor Cells, Cultured
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The in vitro growth requirements of three human embryonal carcinoma cell lines (H 12.7, 2102 EP, 1428 A) were investigated. The basal medium DME/F12 supplemented with insulin, transferrin, and low-density and high-density lipoproteins was sufficient to support substantial multiplication of all three lines. The most efficient attachment factor was either fibronectin (for 2102 EP and 1428 A) or collagen type I (H 12.7). In a serum-free system the influence of epidermal growth factor (EGF), insulin-like growth factor I, multiplication stimulating activity (MSA), a platelet extract, and the glucocorticoids dexamethasone and hydrocortisone, as determined by the DNA synthesis rate of the cells, was generally minimal. However, the DNA synthesis rate of cell lines H 12.7 and 2102 EP was increased by MSA, and the line with the highest potential to differentiate (H 12.7) was stimulated by EGF. All three cell lines secreted growth factors in a heterologous stimulation assay. Insulin-like growth factors I and II were not part of the growth promoting activity. The inhibitory effect of a monoclonal anti-EGF antibody on the 3H-thymidine incorporation of cell line 2102 EP might indicate autocrine secretion of EGF or an EGF-like factor by this cell line.
ISSN:0171-5216
1432-1335
DOI:10.1007/BF00398176