Structural Organization of the rae28 Gene, a Putative Murine Homologue of the Drosophila polyhomeotic Gene

• Published in: Journal of biochemistry (Tokyo) 1996-10, Vol.120 (4), p.797-802
• Main Authors: Motaleb, Md. Abdual, Takihara, Yoshihiro, Nomura, Midori, Matsuda, Yoichi, Higashinakagawa, Toru, Shimada, Kazunori
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Binding Sites; Chromosome Mapping; Cloning, Molecular; DNA, Complementary - genetics; DNA-Binding Proteins - genetics; Drosophila; Drosophila - genetics; Drosophila Proteins; embryogenesis; Exons; F9 cell; Genes, Insect; Genomic Library; genomic organization; Humans; In Situ Hybridization, Fluorescence; Mice; Molecular Sequence Data; Nucleoproteins - genetics; nucleotide sequence; polycomb group genes; Polycomb Repressive Complex 1; Transcription, Genetic
• ISSN: 0021-924X
• DOI: 10.1093/oxfordjournals.jbchem.a021482

A putative murine homologue of the Drosophila polyhomeotic gene, named rae28, has been isolated from a genomic library of 129/SV mouse and its structural organization has been analyzed. rae28 is a single gene of approximately 22 kb long and consists of 15 exons. Its 5′-flanking region lacks typical transcriptional regulatory sequences, such as TATA and CCAAT boxes, but contains GC-rich sequences and seven putative binding sites for a transcription factor, Sp1. One major transcription start point has been determined. The overall exon-intron organization suggested that three different Rae28 mRNAs are generated through alternative splicing. Furthermore, the rae28 gene has been located on the R-positive F3 band of mouse chromosome 6 by the direct R-banding fluorescence in situ hybridization methods.