The theoretical ratio of immunogold labeling of deplasticized epoxy sections and acrylic sections

The purpose of this study was to predict the ratio of immunogold labeling of deplasticized epoxy sections and LR-White sections on the basis of theoretical considerations. Tissues used in the experiments were pancreas, pituitary, kidney, thyroid, and fibrin. Antigens used as test proteins were gluca...

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Bibliographic Details
Published in:Micron (Oxford, England : 1993) England : 1993), 1996-06, Vol.27 (3), p.203-209
Main Authors: Brorson, Sverre-Henning, Skjørten, Fredrik
Format: Article
Language:English
Subjects:
Acrylic resin
Acrylic Resins
Animals
Antigens - analysis
deplasticizing
epoxy resin
Epoxy Resins
Humans
immunocytochemistry
immunoelectron microscopy
immunogold
Immunohistochemistry
LR-White
Rabbits
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Summary:The purpose of this study was to predict the ratio of immunogold labeling of deplasticized epoxy sections and LR-White sections on the basis of theoretical considerations. Tissues used in the experiments were pancreas, pituitary, kidney, thyroid, and fibrin. Antigens used as test proteins were glucagon, somatostatin, thyroglobulin, chromogranin A, ACTH (=Adrenocroticotropt hormone), amyloid A, and fibrinogen. These are proteins of different sizes. The quotient labeling deplasticized/labeling LR-white was deduced theoretically and compared to measurements based on immunogold experiments to obtain a theoretical model with acceptable correlation to the measurements. This study describes a theory—expressed mathematically—for what happens at the molecular level in immunoelectron microscopy at the surface of deplasticized epoxy sections and acrylic sections. The theory explains why we normally get about the same amount of immunogold labeling using LR-White sections (acrylic resin) and deplasticized epoxy sections. Taking the nuances into account, the theory indicates increased usefulness of deplasticized epoxy sections when the diameter of the protein carrying the epitopes decreases.
ISSN:0968-4328
1878-4291
DOI:10.1016/0968-4328(96)00033-9