Chromatographic and electrophoretic applications for the analysis of heparin and dermatan sulfate

Heparin and dermatan sulfate are highly sulfated polydisperse glycosaminoglycans. The methods to determine such compounds include chromatographic and electrophoretic techniques. Here we report on the performances of various analytical methods for the characterization and the determination of GAGs. H...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical applications Biomedical applications, 1996-10, Vol.685 (2), p.223-231
Main Authors: Malsch, Reinhard, Harenberg, Job, Piazolo, Lukas, Huhle, Günther, Heene, Dieter L.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Calibration
Chromatography, Gel - methods
Dalteparin - analysis
Dalteparin - chemistry
Dermatan sulfate
Dermatan Sulfate - analysis
Dermatan Sulfate - chemistry
Electrophoresis, Capillary - methods
Electrophoresis, Polyacrylamide Gel - methods
Fundamental and applied biological sciences. Psychology
Glycoproteins
Heparin
Heparin - analysis
Heparin - chemistry
Molecular Weight
Proteins
Spectrophotometry, Ultraviolet
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Summary:Heparin and dermatan sulfate are highly sulfated polydisperse glycosaminoglycans. The methods to determine such compounds include chromatographic and electrophoretic techniques. Here we report on the performances of various analytical methods for the characterization and the determination of GAGs. Heparin, low-molecular-mass heparins, dermatan sulfate and low-molecular-mass dermatan sulfate were analyzed. High-performance size exclusion chromatography was used to determine the molecular mass, polydispersity, absorbance and the area under the absorbance-time curve. Polyacrylamide gel electrophoresis was used to determine the average molecular mass and the polydispersity. Heparin and dermatan sulfate preparations were analyzed by capillary electrophoresis using reversed polarity. The results obtained reflect different performances of various analytical methods used to characterize GAGs.
ISSN:0378-4347
1572-6495
DOI:10.1016/S0378-4347(96)00185-5