Determination of Z-butylidenephthalide in plasma by high-performance liquid chromatography

• Published in: Journal of chromatography. B, Biomedical applications Biomedical applications, 1996-10, Vol.685 (2), p.379-382
• Main Authors: Ko, Wun-Chang, Yang, Jen-Yen, Chen, Chi-Ming
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Circadian Rhythm; Dose-Response Relationship, Drug; General pharmacology; Injections, Intravenous; Medical sciences; Parasympatholytics - administration & dosage; Parasympatholytics - blood; Parasympatholytics - chemistry; Parasympatholytics - pharmacokinetics; Pharmacology. Drug treatments; Phthalic Anhydrides - administration & dosage; Phthalic Anhydrides - blood; Phthalic Anhydrides - chemistry; Phthalic Anhydrides - pharmacokinetics; Rabbits; Reproducibility of Results; Spectrophotometry, Ultraviolet; Z-Butylidenephthalide
• ISSN: 0378-4347; 1572-6495
• DOI: 10.1016/S0378-4347(96)00201-0

An HPLC assay is described for the determination of Z-butylidenephthalide (Z-Bdph) in plasma. Plasma samples were cleaned up by extraction with 2% chloroform in n-hexane. Z-Bdph was separated on a normal-phase silica column with a mobile phase of chloroform- n-hexane (1:1). The limit of quantitation with UV detection at 254 nm for Z-Bdph in plasma was 0.01 μg/ml. The recovery of Z-Bdph by organic solvent extraction of plasma was 99.5%. The intra-day and inter-day coefficients of variation and relative errors for Z-Bdph determination in plasma were both less than 10%. The present method was applied to pharmacokinetic studies of Z-Bdph in plasma after intravenous administration to rabbits.