Expression of tissue-type plasminogen activator, plasminogen activator inhibitor and von Willebrand factor in the supernatant of endothelial cell cultures in response to the seeding of adenocarcinoma cell line HRT-18

• Published in: Biomedicine & pharmacotherapy 1996, Vol.50 (8), p.373-375
• Main Authors: Morganti, M, Mittermayer, C, Henze, U, Carpi, A, Sagripanti, A
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - chemistry; Adenocarcinoma - pathology; Biological and medical sciences; Coculture Techniques; Endothelium - chemistry; Endothelium, Vascular - chemistry; General aspects (metabolism, cell proliferation, established cell line...); human adenocarcinoma cell culture; human endothelial cell culture; Humans; Medical sciences; PAI-1; Plasminogen Inactivators - analysis; Rectal Neoplasms - chemistry; Rectal Neoplasms - pathology; Tissue Plasminogen Activator - analysis; tPA; Tumor cell; Tumor Cells, Cultured - chemistry; Tumors; Umbilical Veins - chemistry; von Willebrand factor; von Willebrand Factor - analysis
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/S0753-3322(96)89671-5

Plasminogen activator inhibitor (PAI-1), tissue type plasminogen activator (tPA) and von Willebrand factor (vWF) concentrations were measured by ELISA in the supernatant of the following cultures: endothelial cells from human umbilical vein (HUVEC); human colon cancer cells (HRT-18); and coculture cells of HUVEC + HRT-18. No measurable amount of the three substances was found in the supernatant of HRT-18 cell culture. Compared to the value in the HUVEC supernatant, in the UVEC/HRT-18 co-cultures, tPA concentration was significantly lower ( P = 0.0047), PAI-1 significantly higher ( P = 0.026) and vWF also significantly higher ( P = 0.0048). These data indicate that HRT-18 tumor cells do not produce tPA, PAI-1 and vWF; however, these tumor cells induce endothelial cells to change the production of these substances. As a consequence, the interaction between tumor and endothelial cells in vivo may lead to depression of fibrinolysis and enhancement of platelet adhesion.