PCR differentiation of commercial yeast strains using intron splice site primers

The increased use of pure starter cultures in the wine industry has made it necessary to develop a rapid and simple identification system for yeast strains. A method based upon the PCR using oligonucleotide primers that are complementary to intron splice sites has been developed. Since most introns...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1996-12, Vol.62 (12), p.4514-4520
Main Authors: De Barros Lopes, M. (The University of Adelaide, Glen Osmond, Australia.), Soden, A, Henschke, P.A, Langridge, P
Format: Article
Language:English
Subjects:
ADN
AUSTRALIA
AUSTRALIE
Biological and medical sciences
CHIMIOTAXONOMIE
CULTIVOS INICIADORES
CULTURE STARTER
DIFERENCIAS BIOLOGICAS
DIFFERENCE BIOLOGIQUE
DNA Primers
DNA, Fungal - chemistry
ENDOMYCETALES
Fermented food industries
Food industries
Fundamental and applied biological sciences. Psychology
IDENTIFICACION
IDENTIFICATION
Introns
LEVADURA
LEVURE
Microbiology
PCR
POLIMORFISMO GENETICO
Polymerase Chain Reaction
POLYMORPHISME GENETIQUE
QUIMIOTAXONOMIA
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - classification
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
VIN
VINOS
Wine
Wines and vinegars
Yeast
Yeasts - classification
Yeasts - genetics
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Description
Summary:The increased use of pure starter cultures in the wine industry has made it necessary to develop a rapid and simple identification system for yeast strains. A method based upon the PCR using oligonucleotide primers that are complementary to intron splice sites has been developed. Since most introns are not essential for gene function, introns have evolved with minimal constraint. By targeting these highly variable sequences, the PCR has proved to be very effective in uncovering polymorphisms in commercial yeast strains. The speed of the method and the ability to analyze many samples in a single day permit the monitoring of specific yeast strains during fermentations. Furthermore, the simplicity of the technique, which does not require the isolation of DNA, makes it accessible to industrial laboratories that have limited molecular expertise and resources
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.62.12.4514-4520.1996