Enzyme-linked immunosorbent assay, immunoblot analysis and RAST fluoroimmunoassay analysis of serum responses against crude larval antigens of Anisakis simplex in a Spanish random population

The results obtained in a study of seroprevalence by means of ELISA and immunoblot with crude larval extracts of Anisakis simplex using 1008 human sera from Spanish people showing no clinical suspicion of anisakidosis are given. For the evaluation of the results obtained by ELISA the Diagnostic Inde...

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Bibliographic Details
Published in:Journal of helminthology 1996-12, Vol.70 (4), p.281-289
Main Authors: García-Palacios, L., González, M.L., Esteban, M.I., Mirabent, E., Perteguer, M.J., Cuéllar, C.
Format: Article
Language:English
Subjects:
Adult
Animals
anisakiasis
Anisakiasis - epidemiology
Anisakiasis - immunology
Anisakis - immunology
Anisakis simplex
Antibodies, Helminth - blood
antigens
antiserum
Biological and medical sciences
chemiluminescence immunoassays
Child, Preschool
Diseases caused by nematodes
Enzyme-Linked Immunosorbent Assay
extracts
Helminthic diseases
Humans
Immunoblotting
immunoglobulin E
Immunoglobulin E - blood
Infant
Infectious diseases
Medical sciences
Mice
Miscellaneous
nematode larvae
Parasitic diseases
Prevalence
Radioallergosorbent Test
raw fish
raw seafoods
Research Papers
seafoods
seroprevalence
Spain - epidemiology
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Summary:The results obtained in a study of seroprevalence by means of ELISA and immunoblot with crude larval extracts of Anisakis simplex using 1008 human sera from Spanish people showing no clinical suspicion of anisakidosis are given. For the evaluation of the results obtained by ELISA the Diagnostic Index (DI) was used, as the ratio between the optical density resulting from the test serum and the optical density of the negative control. Forty-seven sera showed DIs between 1.5 and 2, and 14 sera were greater than 2. After comparison of the immunoblot analysis with the immunorecognition pattern of a human anisakidosis reference serum, a diagnostic criterion could be established for those sera that, at a 1/100 dilution, showed a DI by ELISA greater than 1.5. Seven of 14 selected sera with DIs in ELISA higher than 1.3 showed anti-Anisakis specific IgE antibodies by RAST fluoroimmunoassay.
ISSN:0022-149X
1475-2697
DOI:10.1017/S0022149X0001556X