Molecular Cloning and Expression Analysis of the Human Rab7 GTP-ase Complementary Deoxyribonucleic Acid

Rab7 is a small GTP-ase localized on late endosomes, which regulates late endocytic membrane traffic in mammalian cells. Moreover it has been shown that this protein has a fundamental role in the cellular vacuolation induced by the cytotoxin VacA ofHelicobacter pylori.We report here for the first ti...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-12, Vol.229 (3), p.887-890
Main Authors: Vitelli, R., Chiariello, M., Lattero, D., Bruni, C.B., Bucci, C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cloning, Molecular
DNA, Complementary - genetics
DNA, Complementary - isolation & purification
Dogs
Female
GTP-Binding Proteins - biosynthesis
GTP-Binding Proteins - genetics
Humans
Mice
Molecular Sequence Data
Placenta
Pregnancy
rab GTP-Binding Proteins
Rats
Sequence Alignment
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Summary:Rab7 is a small GTP-ase localized on late endosomes, which regulates late endocytic membrane traffic in mammalian cells. Moreover it has been shown that this protein has a fundamental role in the cellular vacuolation induced by the cytotoxin VacA ofHelicobacter pylori.We report here for the first time the isolation of a cDNA encoding human Rab7 from a placenta cDNA library. The open reading frame for human Rab7 encodes a protein of 207 amino acids which exhibits high homology with the mouse, rat, and dog counterparts. Northern blot analysis of total RNAs isolated from different cell lines with a cDNA probe containing the entire open reading frame revealed two mRNA transcripts of 2.5 and 1.8 kilobases. The isolation of human Rab7 cDNA will allow further characterization of its function in normal and pathological states.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.1897