Development of a bacterial model for studying anthracycline-membrane interactions

Abstract Growth of wild-type Escherichia coli strain MRE600 was severely affected up to 9 h following treatment with the anthracycline doxorubicin (15 µM), however, after 9 h, the cells became resistant. The onset of resistance coincided with some changes in the relative proportions of total saturat...

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Bibliographic Details
Published in:FEMS microbiology letters 1996-12, Vol.145 (2), p.281-286
Main Authors: Burrow, Shuna M., Rolph, Carole E., Phoenix, David A.
Format: Article
Language:English
Subjects:
Anthracycline
Antibiotics, Antineoplastic - pharmacology
Cell Membrane - drug effects
Cyclopropane
Doxorubicin
Doxorubicin - pharmacology
Drug Resistance, Multiple
E coli
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - metabolism
Fatty acids
Fatty Acids - metabolism
Isopropyl Thiogalactoside - pharmacology
Lipids
Membrane
Membranes
Microbiology
Multidrug resistance
Phospholipid
Phospholipids
Phospholipids - metabolism
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Summary:Abstract Growth of wild-type Escherichia coli strain MRE600 was severely affected up to 9 h following treatment with the anthracycline doxorubicin (15 µM), however, after 9 h, the cells became resistant. The onset of resistance coincided with some changes in the relative proportions of total saturated, monounsaturated and cyclopropane fatty acids. The anionic lipid content in E. coli strain HDL11 is under lac control and synthesis can be induced by incubation with the lac inducer IPTG. HDL11, with low levels of anionic phospholipid, was unaffected by doxorubicin (100 µM) over 9 h, with only slight inhibition of growth seen over 24 h. When the anionic lipid content of HDL11 was increased, there was a slight increase in the efficacy of doxorubicin, providing evidence for a membrane-based step in doxorubicin action.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1996.tb08590.x