Validation of a ribosomal DNA-polymerase chain reaction species diagnostic assay for the common malaria mosquito (Diptera: Culicidae) sibling species complex

A polymerase chain reaction method for identifying individuals in the Anopheles quadrimaculatus Say sibling species complex was validated for wild mosquitoes from Louisiana and Mississippi. This method distinguished An. quadrimaculatus species A, B, C, and D by detecting species-specific differences...

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Bibliographic Details
Published in:Journal of medical entomology 1996-11, Vol.33 (6), p.952-954
Main Authors: Rutledge, C.R. (Louisiana State University, Baton Rouge, LA.), Cornel, A.J, Meek, C.L, Collins, F.H
Format: Article
Language:English
Subjects:
ADN
Animals
Anopheles - genetics
ANOPHELES QUADRIMACULATUS
Biological and medical sciences
CHIMIOTAXONOMIE
Culicidae
DNA, Ribosomal - analysis
ESPECE
ESPECIES
FRATRIE
Freshwater
Fundamental and applied biological sciences. Psychology
HERMANOS
IDENTIFICACION
IDENTIFICATION
LOUISIANE
LUISIANA
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
MISSISSIPPI
PCR
Polymerase Chain Reaction - methods
QUIMIOTAXONOMIA
RIBOSOMAS
RIBOSOME
Vectors. Intermediate hosts
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Summary:A polymerase chain reaction method for identifying individuals in the Anopheles quadrimaculatus Say sibling species complex was validated for wild mosquitoes from Louisiana and Mississippi. This method distinguished An. quadrimaculatus species A, B, C, and D by detecting species-specific differences in the 2nd internal transcribed spacer of ribosomal DNA and was 100% specific and 95% sensitive
ISSN:0022-2585
1938-2928
DOI:10.1093/jmedent/33.6.952