Human-avian influenza virus reassortants: effect of reassortment pattern on multi-cycle reproduction in MDCK cells

Human-avian influenza reassortants possessing the HA gene of the avian parent virus were tested for their ability to replicate in MDCK cells at 37 degrees C and 31 degrees C. Both avian parent viruses, A/Duck/Ukraine/1/63 (H3N8) and A/Duck/Hoshimin/014/78 (H5N3) induced an efficient multi-cycle infe...

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Bibliographic Details
Published in:Archives of virology 1988-01, Vol.103 (1-2), p.117-126
Main Authors: KAVERIN, N. V, RUDNEVA, I. A, SMIRNOV, Y. A, FINSKAYA, N. N
Format: Article
Language:English
Subjects:
Animals
Aves
Biological and medical sciences
Birds - microbiology
Chick Embryo
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetics
Hemagglutinins, Viral - biosynthesis
Hemagglutinins, Viral - genetics
Hemagglutinins, Viral - physiology
Humans
Influenza A virus - genetics
Influenza A virus - pathogenicity
Microbiology
RNA, Viral - analysis
Temperature
Transfection
Viral Proteins - biosynthesis
Virology
Virus Replication
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Summary:Human-avian influenza reassortants possessing the HA gene of the avian parent virus were tested for their ability to replicate in MDCK cells at 37 degrees C and 31 degrees C. Both avian parent viruses, A/Duck/Ukraine/1/63 (H3N8) and A/Duck/Hoshimin/014/78 (H5N3) induced an efficient multi-cycle infection at 37 degrees C, but replicated poorly at 31 degrees C, whereas the human parent virus, MDCK-adapted variant of A/USSR/90/77 (H1N1) strain, replicated efficiently at both temperatures. The reassortant clone possessing the HA gene of A/Duck/Ukraine/1/63 virus and the other 7 genes of A/USSR/90/77 virus replicated at both temperatures almost as efficiently as the human parent virus. Among the reassortants between A/Duck/Hoshimin/014/78 and A/USSR/90/77, the clones possessing the HA and NA genes of the avian strain, or the HA, NA, NP, and NS genes of the avian strain, and the other genes of the human parent virus, replicated poorly at both temperatures, especially at 31 degrees C, whereas the reassortant possessing the HA, NA, and M genes of the avian virus replicated at both temperatures fairly efficiently. The results are discussed in connection with the limitations imposed by different genes upon avian influenza viruses' ability to replicate in mammalian cells.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01319813