Comparison of human and Xenopus GATA-2 promoters

GATA proteins comprise a family of transcription factors that are required for appropriate development of hematopoietic lineages. In order to understand the transcriptional regulation of GATA genes, we have cloned the human GATA-2 gene and identified and characterized its promoter. Comparison with t...

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Bibliographic Details
Published in:Gene 1996-11, Vol.179 (2), p.219-223
Main Authors: Fleenor, Donald E., Langdon, Scott D., deCastro, Carlos M., Kaufman, Russel E.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
CCAAT factors
Cloning
Cloning, Molecular
Conserved Sequence
DNA
DNA-Binding Proteins - genetics
Erythropoiesis
Ets factors
GATA2 Transcription Factor
Gene
Humans
Molecular Sequence Data
Promoter Regions, Genetic
Sequence Homology, Nucleic Acid
Transcription Factors - genetics
Xenopus
Xenopus Proteins
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Summary:GATA proteins comprise a family of transcription factors that are required for appropriate development of hematopoietic lineages. In order to understand the transcriptional regulation of GATA genes, we have cloned the human GATA-2 gene and identified and characterized its promoter. Comparison with the Xenopus GATA-2 promoter demonstrates highly conserved CCAAT box elements, which are essential for appropriate Xenopus expression. Unlike the Xenopus gene, the human GATA-2 gene lacks GATA binding motifs within the first 800 bp of 5′ flanking sequence. In addition, the human GATA-2 promoter has two highly conserved ets sites that resemble the binding site for a recently described ets repressor factor, ERF. These conserved DNA sequence motifs provide strong candidate regions for the regulation of GATA-2.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(96)00355-1