Cloning and characterization of femA and femB from Staphylococcus epidermidis

A DNA fragment was identified and cloned from Staphylococcus epidermidis (Se) using femA from S. aureus (Sa) as a heterologous hybridization probe. DNA sequence analysis of a portion of this clone revealed two complete ORFs highly related to femA and femB of Sa. The genomic arrangement of the Se fem...

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Bibliographic Details
Published in:Gene 1996-11, Vol.180 (1), p.177-181
Main Authors: Alborn, William E., Hoskins, Joann, Ünal, Serhat, Flokowitsch, Jane E., Hayes, Carolyn A., Dotzlaf, Joe E., Yeh, W.K., Skatrud, Paul L.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Proteins - genetics
Bacterial Proteins - isolation & purification
Cloning
Cloning, Molecular
DNA, Bacterial
Escherichia coli
FEMA purification
Genes, Bacterial
Methicillin resistance
Molecular Sequence Data
Nucleotide sequencing
Plasmids
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - isolation & purification
Sequence Homology, Amino Acid
Staphylococcus epidermidis
Staphylococcus epidermidis - genetics
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Summary:A DNA fragment was identified and cloned from Staphylococcus epidermidis (Se) using femA from S. aureus (Sa) as a heterologous hybridization probe. DNA sequence analysis of a portion of this clone revealed two complete ORFs highly related to femA and femB of Sa. The genomic arrangement of the Se femA/B complex was nearly identical to that observed in Sa. Intra- and interspecies relatedness of these genes and conservation of genomic organization were consistent with gene duplication of one of these genes in an ancestral organism. Recombinant FEMA, produced in Escherichia coli (Ec), was purified to near homogeneity. Identity of the purified protein was verified by N-terminal amino acid (aa) sequence analysis.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(96)00450-7