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Cloning and characterization of femA and femB from Staphylococcus epidermidis
A DNA fragment was identified and cloned from Staphylococcus epidermidis (Se) using femA from S. aureus (Sa) as a heterologous hybridization probe. DNA sequence analysis of a portion of this clone revealed two complete ORFs highly related to femA and femB of Sa. The genomic arrangement of the Se fem...
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| Published in: | Gene 1996-11, Vol.180 (1), p.177-181 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c391t-79e741cfb65f4ec529315b9a34d6ee90f103dde5291d6707cbae557c73a8b5423 |
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| cites | cdi_FETCH-LOGICAL-c391t-79e741cfb65f4ec529315b9a34d6ee90f103dde5291d6707cbae557c73a8b5423 |
| container_end_page | 181 |
| container_issue | 1 |
| container_start_page | 177 |
| container_title | Gene |
| container_volume | 180 |
| creator | Alborn, William E. Hoskins, Joann Ünal, Serhat Flokowitsch, Jane E. Hayes, Carolyn A. Dotzlaf, Joe E. Yeh, W.K. Skatrud, Paul L. |
| description | A DNA fragment was identified and cloned from
Staphylococcus epidermidis (Se) using
femA from
S. aureus (Sa) as a heterologous hybridization probe. DNA sequence analysis of a portion of this clone revealed two complete ORFs highly related to
femA and
femB of
Sa. The genomic arrangement of the
Se femA/B complex was nearly identical to that observed in
Sa. Intra- and interspecies relatedness of these genes and conservation of genomic organization were consistent with gene duplication of one of these genes in an ancestral organism. Recombinant FEMA, produced in
Escherichia coli (Ec), was purified to near homogeneity. Identity of the purified protein was verified by N-terminal amino acid (aa) sequence analysis. |
| doi_str_mv | 10.1016/S0378-1119(96)00450-7 |
| format | article |
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Staphylococcus epidermidis (Se) using
femA from
S. aureus (Sa) as a heterologous hybridization probe. DNA sequence analysis of a portion of this clone revealed two complete ORFs highly related to
femA and
femB of
Sa. The genomic arrangement of the
Se femA/B complex was nearly identical to that observed in
Sa. Intra- and interspecies relatedness of these genes and conservation of genomic organization were consistent with gene duplication of one of these genes in an ancestral organism. Recombinant FEMA, produced in
Escherichia coli (Ec), was purified to near homogeneity. Identity of the purified protein was verified by N-terminal amino acid (aa) sequence analysis.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(96)00450-7</identifier><identifier>PMID: 8973364</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - genetics ; Bacterial Proteins - isolation & purification ; Cloning ; Cloning, Molecular ; DNA, Bacterial ; Escherichia coli ; FEMA purification ; Genes, Bacterial ; Methicillin resistance ; Molecular Sequence Data ; Nucleotide sequencing ; Plasmids ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - isolation & purification ; Sequence Homology, Amino Acid ; Staphylococcus epidermidis ; Staphylococcus epidermidis - genetics</subject><ispartof>Gene, 1996-11, Vol.180 (1), p.177-181</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-79e741cfb65f4ec529315b9a34d6ee90f103dde5291d6707cbae557c73a8b5423</citedby><cites>FETCH-LOGICAL-c391t-79e741cfb65f4ec529315b9a34d6ee90f103dde5291d6707cbae557c73a8b5423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8973364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alborn, William E.</creatorcontrib><creatorcontrib>Hoskins, Joann</creatorcontrib><creatorcontrib>Ünal, Serhat</creatorcontrib><creatorcontrib>Flokowitsch, Jane E.</creatorcontrib><creatorcontrib>Hayes, Carolyn A.</creatorcontrib><creatorcontrib>Dotzlaf, Joe E.</creatorcontrib><creatorcontrib>Yeh, W.K.</creatorcontrib><creatorcontrib>Skatrud, Paul L.</creatorcontrib><title>Cloning and characterization of femA and femB from Staphylococcus epidermidis</title><title>Gene</title><addtitle>Gene</addtitle><description>A DNA fragment was identified and cloned from
Staphylococcus epidermidis (Se) using
femA from
S. aureus (Sa) as a heterologous hybridization probe. DNA sequence analysis of a portion of this clone revealed two complete ORFs highly related to
femA and
femB of
Sa. The genomic arrangement of the
Se femA/B complex was nearly identical to that observed in
Sa. Intra- and interspecies relatedness of these genes and conservation of genomic organization were consistent with gene duplication of one of these genes in an ancestral organism. Recombinant FEMA, produced in
Escherichia coli (Ec), was purified to near homogeneity. Identity of the purified protein was verified by N-terminal amino acid (aa) sequence analysis.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>DNA, Bacterial</subject><subject>Escherichia coli</subject><subject>FEMA purification</subject><subject>Genes, Bacterial</subject><subject>Methicillin resistance</subject><subject>Molecular Sequence Data</subject><subject>Nucleotide sequencing</subject><subject>Plasmids</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - isolation & purification</subject><subject>Sequence Homology, Amino Acid</subject><subject>Staphylococcus epidermidis</subject><subject>Staphylococcus epidermidis - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkMtOwzAQRS0EKqXwCZWyQrAI2HFsxytUKl5SEYvC2nLsCTVK4mKnSOXrSR9i29nMaO6dudJBaEzwDcGE384xFUVKCJFXkl9jnDOciiM0JIWQKca0OEbDf8spOovxC_fFWDZAg0IKSnk-RK_T2reu_Ux0axOz0EGbDoL71Z3zbeKrpIJmshX74T6pgm-SeaeXi3XtjTdmFRNYOguhcdbFc3RS6TrCxb6P0Mfjw_v0OZ29Pb1MJ7PUUEm6VEgQOTFVyVmVg2GZpISVUtPccgCJK4KptdDvieUCC1NqYEwYQXVRsjyjI3S5-7sM_nsFsVONiwbqWrfgV1GJgtM8I_ygkTAhM9HnjxDbGU3wMQao1DK4Roe1IlhteKstb7WBqSRXW95K9HfjfcCqbMD-X-0B9_rdTocex4-DoKJx0BqwLoDplPXuQMIfjWuPVg</recordid><startdate>19961121</startdate><enddate>19961121</enddate><creator>Alborn, William E.</creator><creator>Hoskins, Joann</creator><creator>Ünal, Serhat</creator><creator>Flokowitsch, Jane E.</creator><creator>Hayes, Carolyn A.</creator><creator>Dotzlaf, Joe E.</creator><creator>Yeh, W.K.</creator><creator>Skatrud, Paul L.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19961121</creationdate><title>Cloning and characterization of femA and femB from Staphylococcus epidermidis</title><author>Alborn, William E. ; Hoskins, Joann ; Ünal, Serhat ; Flokowitsch, Jane E. ; Hayes, Carolyn A. ; Dotzlaf, Joe E. ; Yeh, W.K. ; Skatrud, Paul L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-79e741cfb65f4ec529315b9a34d6ee90f103dde5291d6707cbae557c73a8b5423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>DNA, Bacterial</topic><topic>Escherichia coli</topic><topic>FEMA purification</topic><topic>Genes, Bacterial</topic><topic>Methicillin resistance</topic><topic>Molecular Sequence Data</topic><topic>Nucleotide sequencing</topic><topic>Plasmids</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - isolation & purification</topic><topic>Sequence Homology, Amino Acid</topic><topic>Staphylococcus epidermidis</topic><topic>Staphylococcus epidermidis - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alborn, William E.</creatorcontrib><creatorcontrib>Hoskins, Joann</creatorcontrib><creatorcontrib>Ünal, Serhat</creatorcontrib><creatorcontrib>Flokowitsch, Jane E.</creatorcontrib><creatorcontrib>Hayes, Carolyn A.</creatorcontrib><creatorcontrib>Dotzlaf, Joe E.</creatorcontrib><creatorcontrib>Yeh, W.K.</creatorcontrib><creatorcontrib>Skatrud, Paul L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alborn, William E.</au><au>Hoskins, Joann</au><au>Ünal, Serhat</au><au>Flokowitsch, Jane E.</au><au>Hayes, Carolyn A.</au><au>Dotzlaf, Joe E.</au><au>Yeh, W.K.</au><au>Skatrud, Paul L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of femA and femB from Staphylococcus epidermidis</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1996-11-21</date><risdate>1996</risdate><volume>180</volume><issue>1</issue><spage>177</spage><epage>181</epage><pages>177-181</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A DNA fragment was identified and cloned from
Staphylococcus epidermidis (Se) using
femA from
S. aureus (Sa) as a heterologous hybridization probe. DNA sequence analysis of a portion of this clone revealed two complete ORFs highly related to
femA and
femB of
Sa. The genomic arrangement of the
Se femA/B complex was nearly identical to that observed in
Sa. Intra- and interspecies relatedness of these genes and conservation of genomic organization were consistent with gene duplication of one of these genes in an ancestral organism. Recombinant FEMA, produced in
Escherichia coli (Ec), was purified to near homogeneity. Identity of the purified protein was verified by N-terminal amino acid (aa) sequence analysis.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8973364</pmid><doi>10.1016/S0378-1119(96)00450-7</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1996-11, Vol.180 (1), p.177-181 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78634216 |
| source | Elsevier |
| subjects | Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Cloning Cloning, Molecular DNA, Bacterial Escherichia coli FEMA purification Genes, Bacterial Methicillin resistance Molecular Sequence Data Nucleotide sequencing Plasmids Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification Sequence Homology, Amino Acid Staphylococcus epidermidis Staphylococcus epidermidis - genetics |
| title | Cloning and characterization of femA and femB from Staphylococcus epidermidis |
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