SHP-2 Is Involved in Neurotrophin Signaling

To investigate the involvement of SHP-2 in the signal transduction pathway stimulated by neurotrophins, the association of SHP-2 with components of the pathway was examined. Following NGF stimulation of PC12 cells, SHP-2 was found to be associated with the p85 subunit of PI3-kinase and the Shc prote...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-12, Vol.229 (2), p.607-611
Main Authors: Okada, Noriko, Wada, Kazuyo, Goldsmith, Bruce A., Koizumi, Shinichi
Format: Article
Language:English
Subjects:
Animals
Brain-Derived Neurotrophic Factor - metabolism
Cell Line
Intracellular Signaling Peptides and Proteins
Nerve Growth Factors - metabolism
PC12 Cells
Phosphorylation
Precipitin Tests
Protein Tyrosine Phosphatase, Non-Receptor Type 11
Protein Tyrosine Phosphatase, Non-Receptor Type 6
Protein Tyrosine Phosphatases - metabolism
Rats
Signal Transduction
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Summary:To investigate the involvement of SHP-2 in the signal transduction pathway stimulated by neurotrophins, the association of SHP-2 with components of the pathway was examined. Following NGF stimulation of PC12 cells, SHP-2 was found to be associated with the p85 subunit of PI3-kinase and the Shc proteins. In retinoic acid-differentiated SH-SY5Y cells and primary cultures of rat cortical neurons, BDNF treatment similarly caused the association of SHP-2 with p85. In addition, a tyrosine-phosphorylated protein, which is probably TrkB, was coimmunoprecipitated with SHP-2 in both cultures. These results show that SHP-2 becomes associated with signaling proteins after treatment with neurotrophins and suggest that SHP-2 plays a fundamental role in neurotrophin signaling.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.1851