SHP-2 Is Involved in Neurotrophin Signaling

• Published in: Biochemical and biophysical research communications 1996-12, Vol.229 (2), p.607-611
• Main Authors: Okada, Noriko, Wada, Kazuyo, Goldsmith, Bruce A., Koizumi, Shinichi
• Format: Article
• Language: English
• Subjects: Animals; Brain-Derived Neurotrophic Factor - metabolism; Cell Line; Intracellular Signaling Peptides and Proteins; Nerve Growth Factors - metabolism; PC12 Cells; Phosphorylation; Precipitin Tests; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Protein Tyrosine Phosphatase, Non-Receptor Type 6; Protein Tyrosine Phosphatases - metabolism; Rats; Signal Transduction
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1996.1851

To investigate the involvement of SHP-2 in the signal transduction pathway stimulated by neurotrophins, the association of SHP-2 with components of the pathway was examined. Following NGF stimulation of PC12 cells, SHP-2 was found to be associated with the p85 subunit of PI3-kinase and the Shc proteins. In retinoic acid-differentiated SH-SY5Y cells and primary cultures of rat cortical neurons, BDNF treatment similarly caused the association of SHP-2 with p85. In addition, a tyrosine-phosphorylated protein, which is probably TrkB, was coimmunoprecipitated with SHP-2 in both cultures. These results show that SHP-2 becomes associated with signaling proteins after treatment with neurotrophins and suggest that SHP-2 plays a fundamental role in neurotrophin signaling.