Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross-hybridization and restriction analysis. Sequ...

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Bibliographic Details
Published in:Plant molecular biology 1996-11, Vol.32 (4), p.759-765
Main Authors: Utsugi, S. (Okayama Univ., Kurashiki (Japan). Research Inst. for Bioresources), Sakamoto, W, Ogura, Y, Murata, M, Motoyoshi, F
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - genetics
ARABIDOPSIS THALIANA
ARN MENSAJERO
ARN MESSAGER
CELL MEMBRANES
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning, Molecular
DNA HYBRIDIZATION
DNA, Complementary - genetics
DNA, Plant - genetics
EXPRESION GENICA
EXPRESSION DES GENES
FLEUR
FLORES
FLOWERS
GENE
GENE EXPRESSION
Gene Expression Regulation, Plant - physiology
GENES
HIBRIDACION DE ADN
HYBRIDATION D'ADN
MEMBRANAS CELULARES
MEMBRANE CELLULAIRE
MESSENGER RNA
MOLECULAR CLONING
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
Plant Leaves - chemistry
PROTEINAS
PROTEINAS DE RESERVA
PROTEINE
PROTEINE DE RESERVE
PROTEINS
RNA, Messenger - analysis
SECUENCIA NUCLEOTIDICA
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
SEQUENCE NUCLEOTIDIQUE
STORAGE PROTEINS
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Summary:Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross-hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00020217