Acute Expression of the PRL Receptor Gene after Ovariectomy in Midpregnant Mouse Mammary Gland

In order to examine the time-dependent expression of the PRL receptor (PRL-R) gene at lactogenesis, the level of PRL-R mRNA was determined following ovariectomy in pregnant mouse mammary gland. Following reverse transcription, the quantity of mRNA was measured by the competitive polymerase chain rea...

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Bibliographic Details
Published in:Endocrine Journal 1996, Vol.43(5), pp.537-544
Main Authors: MIZOGUCHI, YASUSI, KIM, JAE YOUNG, SASAKI, TETSUO, HAMA, TARO, SASAKI, MASATO, ENAMI, JUMPEI, SAKAI, SENKITI
Format: Article
Language:English
Subjects:
Adrenalectomy
Animals
Base Sequence
Casein mRNA
Caseins - analysis
Caseins - genetics
Caseins - metabolism
DNA Primers - chemistry
Female
Gene Expression Regulation - genetics
Lactogenesis
Mammary gland
Mammary Glands, Animal - chemistry
Mammary Glands, Animal - metabolism
Mice
Mice, Inbred ICR
Ovariectomy
Polymerase Chain Reaction
Pregnancy
PRL receptor mRNA
Receptors, Prolactin - analysis
Receptors, Prolactin - classification
Receptors, Prolactin - genetics
Receptors, Prolactin - metabolism
RNA, Messenger - analysis
RNA, Messenger - genetics
Time Factors
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Summary:In order to examine the time-dependent expression of the PRL receptor (PRL-R) gene at lactogenesis, the level of PRL-R mRNA was determined following ovariectomy in pregnant mouse mammary gland. Following reverse transcription, the quantity of mRNA was measured by the competitive polymerase chain reaction. The casein (a 22000 molecular weight component) mRNA level was measured as a marker for milk synthesis. Following ovariectomy, the onset of abortion occurred mostly at 22-23h and the level of casein mRNA began to increase at 12h. The long and short forms of PRL-R mRNAs were detected in a molar ratio of 1:0.2 on day 12 of pregnancy. Eight h after ovariectomy, the long form of PRL-R mRNA began to increase, showing a bell-shaped profile with the highest peak at 16h. The short form of PRL-R mRNA was at low levels and remained constant. The levels of the long form of PRL-R mRNA decreased similarly in the presence and absence of foster pups from 24 to 48h. Conversely, casein mRNA were maintained at high levels by supplying foster pups. The level of the long form of PRL-R mRNA reached a maximum prior to abortion. The present experiments demonstrated that the acute expression of the PRL-R gene occurred in the mammary gland at lactogenesis.
ISSN:0918-8959
1348-4540
DOI:10.1507/endocrj.43.537