Effect of platelet-activating factor on leukocyte adhesion to microvascular endothelium. Time course and dose-response relationships

The hamster cheek pouch microcirculation was used to investigate the effects of platelet-activating factor (PAF) on leukocyte adhesion to microvascular walls by means of intravital microscopy. PAF was applied topically at concentrations ranging from 10(-11) to 10(-5) M. An inverse relationship betwe...

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Bibliographic Details
Published in:Inflammation 1988-12, Vol.12 (6), p.563-573
Main Authors: DILLON, P. K, FITZPATRICK, M. F, RITTER, A. B, DURAN, W. N
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Adhesion - drug effects
Cricetinae
Dose-Response Relationship, Drug
Endothelium, Vascular - drug effects
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Leukocytes - drug effects
Male
Mesocricetus
Myeloid cells: ontogeny, maturation, markers, receptors
Platelet Activating Factor - pharmacology
Time Factors
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Summary:The hamster cheek pouch microcirculation was used to investigate the effects of platelet-activating factor (PAF) on leukocyte adhesion to microvascular walls by means of intravital microscopy. PAF was applied topically at concentrations ranging from 10(-11) to 10(-5) M. An inverse relationship between PAF concentration and number of adhering white cells per 100-microns length was found in venules ranging in diameter from 10 to 60 microns (grouped into 10-microns intervals). Importantly, the PAF-induced adhesion of leukocytes lasted for the 3-h experimental period. We postulate that induction of leukocyte adhesion to venular endothelium is an important role of PAF in inflammatory processes.
ISSN:0360-3997
1573-2576
DOI:10.1007/BF00914318