N-linked sugar chain of 55-kDa royal jelly glycoprotein

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1996-12, Vol.60 (12), p.2099-2102
• Main Authors: Kimura, Y. (Okayama Univ. (Japan). Faculty of Agriculture), Kajiyama, S, Kanaeda, J, Izukawa, T, Yonekura, M
• Format: Article
• Language: English
• Subjects: Animals; Apidae; Apis mellifera; Bees - metabolism; Biological and medical sciences; Biotechnology; Carbohydrate Sequence; CHEMICAL STRUCTURE; Chromatography, High Pressure Liquid; ESTRUCTURA QUIMICA; Fatty Acids - chemistry; GELEE ROYALE; General pharmacology; GLICOPROTEINAS; GLYCOPROTEINE; GLYCOPROTEINS; Glycoproteins - chemistry; Glycoproteins - isolation & purification; insect glycoprotein; Insect Hormones - chemistry; JALEA REAL; Magnetic Resonance Spectroscopy; MALDI-TOF MS; Medical sciences; Molecular Sequence Data; N-glycan; PA-sugar chain; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; POLISACARIDOS; POLYHOLOSIDE; POLYSACCHARIDES; ROYAL JELLY; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; STRUCTURE CHIMIQUE
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.60.2099

An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507-509 (1995)], has been identified. The sugar chains were released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential exo-mannosidase digestions, MALDI-TOF MS, and 500 MHz 1 H-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Manα1→2Manα1→6(Manα1→2Manα1→3)Manαl→6(Manα1→2Manα1→2Manα1 →3)Manβ1→4GlcNAcβl→4GlcNAc, which is a non-processed high mannose type structure.