N-linked sugar chain of 55-kDa royal jelly glycoprotein

An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507-509 (1995)], has been identified. The sugar chains were...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1996-12, Vol.60 (12), p.2099-2102
Main Authors: Kimura, Y. (Okayama Univ. (Japan). Faculty of Agriculture), Kajiyama, S, Kanaeda, J, Izukawa, T, Yonekura, M
Format: Article
Language:English
Subjects:
Animals
Apidae
Apis mellifera
Bees - metabolism
Biological and medical sciences
Biotechnology
Carbohydrate Sequence
CHEMICAL STRUCTURE
Chromatography, High Pressure Liquid
ESTRUCTURA QUIMICA
Fatty Acids - chemistry
GELEE ROYALE
General pharmacology
GLICOPROTEINAS
GLYCOPROTEINE
GLYCOPROTEINS
Glycoproteins - chemistry
Glycoproteins - isolation & purification
insect glycoprotein
Insect Hormones - chemistry
JALEA REAL
Magnetic Resonance Spectroscopy
MALDI-TOF MS
Medical sciences
Molecular Sequence Data
N-glycan
PA-sugar chain
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
POLISACARIDOS
POLYHOLOSIDE
POLYSACCHARIDES
ROYAL JELLY
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
STRUCTURE CHIMIQUE
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cited_by cdi_FETCH-LOGICAL-c670t-5e0e1c83620dbb8b1b8965dd2e4b83d83b162d5e62013e93e8b534b65479bb713
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container_issue 12
container_start_page 2099
container_title Bioscience, biotechnology, and biochemistry
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creator Kimura, Y. (Okayama Univ. (Japan). Faculty of Agriculture)
Kajiyama, S
Kanaeda, J
Izukawa, T
Yonekura, M
description An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507-509 (1995)], has been identified. The sugar chains were released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential exo-mannosidase digestions, MALDI-TOF MS, and 500 MHz 1 H-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Manα1→2Manα1→6(Manα1→2Manα1→3)Manαl→6(Manα1→2Manα1→2Manα1 →3)Manβ1→4GlcNAcβl→4GlcNAc, which is a non-processed high mannose type structure.
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identifier ISSN: 0916-8451
ispartof Bioscience, biotechnology, and biochemistry, 1996-12, Vol.60 (12), p.2099-2102
issn 0916-8451
1347-6947
language eng
recordid cdi_proquest_miscellaneous_78653121
source Oxford Journals Online; EZB Electronic Journals Library
subjects Animals
Apidae
Apis mellifera
Bees - metabolism
Biological and medical sciences
Biotechnology
Carbohydrate Sequence
CHEMICAL STRUCTURE
Chromatography, High Pressure Liquid
ESTRUCTURA QUIMICA
Fatty Acids - chemistry
GELEE ROYALE
General pharmacology
GLICOPROTEINAS
GLYCOPROTEINE
GLYCOPROTEINS
Glycoproteins - chemistry
Glycoproteins - isolation & purification
insect glycoprotein
Insect Hormones - chemistry
JALEA REAL
Magnetic Resonance Spectroscopy
MALDI-TOF MS
Medical sciences
Molecular Sequence Data
N-glycan
PA-sugar chain
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
POLISACARIDOS
POLYHOLOSIDE
POLYSACCHARIDES
ROYAL JELLY
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
STRUCTURE CHIMIQUE
title N-linked sugar chain of 55-kDa royal jelly glycoprotein
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