Casein kinases I and II bound to pig brain microtubules

1. Microtubules prepared from pig brain by two cycles of assembly-disassembly comprise cyclic nucleotide-independent protein kinase activity with phosvitin and troponin T as substrates. 2. Phosphocellulose chromatography resolved two phosvitin kinase activity peaks, one of which coincided with the t...

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Published in:Cellular and molecular neurobiology 1988-09, Vol.8 (3), p.315-324
Main Authors: RISNIK, V. V, ADAM, G, GUSEV, N. B, FRIEDRICH, P
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Brain - enzymology
Casein Kinases
Contractile proteins
Fundamental and applied biological sciences. Psychology
Holoproteins
Microtubule Proteins - metabolism
Microtubules - enzymology
Phosphorylation
Protein Kinases - metabolism
Proteins
Subcellular Fractions
Swine - metabolism
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Summary:1. Microtubules prepared from pig brain by two cycles of assembly-disassembly comprise cyclic nucleotide-independent protein kinase activity with phosvitin and troponin T as substrates. 2. Phosphocellulose chromatography resolved two phosvitin kinase activity peaks, one of which coincided with the troponin T kinase peak. 3. The activity peak corresponding to troponin T kinase was inhibited by heparin (I50 = 0.06 micrograms/ml), whereas the other phosvitin kinase peak was unaffected. 4. Both kinase fractions phosphorylated tubulin and microtubule-associated protein (MAP-2). 5. It is concluded that pig brain microtubules contain bound casein kinases I and II. The association may target the action of these kinases toward microtubular proteins in vivo.
ISSN:0272-4340
1573-6830
DOI:10.1007/BF00711173