An eye color gene for the detection of transgenic non-drosophilid insects

A genetic marker for identifying transgenic Musca domestica by changes in eye color is described. The Drosophila melanogaster tryptophan oxygenase gene, vermilion (v), was tested for its ability to genetically complement the mutant tryptophan oxygenase gene in houseflies homozygous for green (ge). T...

Full description

Saved in:
Bibliographic Details
Published in:Insect biochemistry and molecular biology 1996-07, Vol.26 (7), p.641-644
Main Authors: White, Lisa D., Coates, Craig J., Atkinson, Peter W., O'Brochta, David A.
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
color
complementary DNA
complementary genes
compound eyes
Drosophila melanogaster
Drosophila melanogaster - enzymology
Eye Color - genetics
eye color mutants
genetic complementation
Genetic Markers
genetic transformation
Genetic Vectors
Houseflies - embryology
Houseflies - genetics
Musca domestica
mutants
Phenotype
promoter regions
structural genes
Transgenes
transgenic animals
transgenic markers
tryptophan 2,3-dioxygenase
Tryptophan Oxygenase - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A genetic marker for identifying transgenic Musca domestica by changes in eye color is described. The Drosophila melanogaster tryptophan oxygenase gene, vermilion (v), was tested for its ability to genetically complement the mutant tryptophan oxygenase gene in houseflies homozygous for green (ge). The v cDNA, placed under the control of the hsp82 promoter of D. pseudoobscura was transiently expressed in M. domestica embryos homozygous for the tryptophan oxygenase gene, ge, resulting in the rescue of adult eye color. The use of a gene from D. melanogaster to complement an eye color mutant in Musca provides the opportunity to develop a gene vector system for M. domestica and a select group of other non-drosophilid insects in which homologous mutations exist.
ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(96)00039-2