Lactobacillus curvatus has a glucose transport system homologous to the mannose family of phosphoenolpyruvate-dependent phosphotransferase systems

In Lactobacillus curvatus, a phosphoenolpyruvate:mannose phosphotransferase system (mannose-PTS) has been characterized and it was shown to be involved in glucose and mannose transport, but no glucose-specific PTS activity could be detected. A 2.1 kb DNA fragment amplified by PCR from the L. curvatu...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 1996-12, Vol.142 (12), p.3469-3477
Main Authors: Veyrat, A, Gosalbes, M.J, Perez-Martinez, G
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteriology
Base Sequence
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Chromosome Mapping
Chromosomes, Bacterial
Cloning, Molecular
DNA Primers
food microbiology
food processing
Fundamental and applied biological sciences. Psychology
genbank/u28163
Genes, Bacterial
Lactobacillus - enzymology
Lactobacillus - genetics
meat products
Microbiology
Mission oriented research
Molecular Sequence Data
Open Reading Frames
Permeability, membrane transport, intracellular transport
Phosphoenolpyruvate Sugar Phosphotransferase System - chemistry
Phosphoenolpyruvate Sugar Phosphotransferase System - genetics
Phosphoenolpyruvate Sugar Phosphotransferase System - metabolism
Phosphorylation
Physiology and metabolism
Polymerase Chain Reaction
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
Sequence Homology, Amino Acid
Substrate Specificity
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Description
Summary:In Lactobacillus curvatus, a phosphoenolpyruvate:mannose phosphotransferase system (mannose-PTS) has been characterized and it was shown to be involved in glucose and mannose transport, but no glucose-specific PTS activity could be detected. A 2.1 kb DNA fragment amplified by PCR from the L. curvatus genome was sequenced. Sequence analysis showed four ORFs which could encode proteins similar to PTS transporters EIIA, EIIB, EIIC and EIID of the mannose class. The expression of the manB gene (encoding EIIB) from L. curvatus in a mutant of Lactobacillus sake impaired in EIIMan activity restored this activity. Furthermore, this DNA fragment complemented the regulatory function of LevE (EIIB) in a Bacillus subtilis levE-deficient mutant, suggesting that the protein encoded by manB could also play a regulatory role in L. curvatus.
ISSN:1350-0872
1465-2080
DOI:10.1099/13500872-142-12-3469