Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression

Analysis of Agrobacterium-transferred DNA (T-DNA) revealed strong correlations between transgene structures and floral pigmentation patterns from chalcone synthase (chs) co-suppression among 47 Petunia transformants. Presented here are the full details of T-DNA structural organization in that popula...

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Bibliographic Details
Published in:Plant molecular biology 1996-12, Vol.32 (6), p.1197-1203
Main Authors: Cluster, P.D. (John Innes Centre, Colney Lane, Norwich (United Kingdom).), O'Dell, M, Metzlaff, M, Flavell, R.B
Format: Article
Language:English
Subjects:
ADN RECOMBINADO
ADN RECOMBINE
AGROBACTERIUM
Blotting, Southern
CALCONA
CHALCONE
CHALCONES
DNA Restriction Enzymes
DNA, Bacterial - analysis
DNA, Bacterial - chemistry
FENOTIPOS
FLEUR
FLORES
FLOWERS
GENE TRANSFER
GENETIC INHERITANCE
Genetic Linkage
Genetic Vectors
HEREDITE
HERENCIA GENETICA
NUCLEOTIDE SEQUENCE
PETUNIA
PHENOTYPE
PHENOTYPES
PIGMENTACION
PIGMENTATION
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified - genetics
RECOMBINANT DNA
Repetitive Sequences, Nucleic Acid
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Suppression, Genetic
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
Transformation, Genetic
TRANSGENIC PLANTS
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Summary:Analysis of Agrobacterium-transferred DNA (T-DNA) revealed strong correlations between transgene structures and floral pigmentation patterns from chalcone synthase (chs) co-suppression among 47 Petunia transformants. Presented here are the full details of T-DNA structural organization in that population. Sixteen transformants (34%) carried one T-DNA copy while 31 (66%) carried 106 complete and partial T-DNA elements in 54 linkage groups. Thirty linkage groups contained multiple T-DNA copies; 15 of these contained only contiguously repeated copies, 8 contained only dispersed copies and 7 contained both. Right-border inverted repeats were three times more frequent than left-border inverted or direct repeats. Large fragments of binary-vector sequences were linked to the T-DNA in seven plants.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00041406