Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20

• Published in: Veterinary immunology and immunopathology 1996-12, Vol.55 (1), p.225-234
• Main Authors: Rothwell, C.J., Vervelde, L., Davison, T.F.
• Format: Article
• Language: English
• Subjects: Alloantigens; ANIMAL MODELS; Animals; Antibodies, Monoclonal - chemistry; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; ANTIGENE; ANTIGENOS; ANTIGENS; Antigens, Differentiation, B-Lymphocyte - analysis; Antigens, Differentiation, B-Lymphocyte - immunology; B cells; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Bu-1; Bursa; Bursa of Fabricius - immunology; Bursa of Fabricius - metabolism; Chicken; CHICKENS; Flow Cytometry; Immunohistochemistry; MODELE ANIMAL; MODELOS ANIMALES; MONOCLONAL ANTIBODIES; POLLO; POULET; Precipitin Tests - veterinary
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/S0165-2427(96)05635-8

The genetically polymorphic chicken antigen Bu-1 (chB6) has been identified by alloantisera raised against RPL line 6 3 (Bu-1a) and line 7 2 (Bu-1b) birds and subsequently by monoclonal antibodies (mAbs) which identify individual alloantigens. We have produced a monoclonal antibody, AV20, which recognises a monomorphic determinant on the antigen Bu-1. AV20 identifies a marker on both bursal and peripheral B cells. Staining characteristics on bursa, spleen, thymus and peripheral blood lymphocytes are similar to those of the allotypic antibodies which identify Bu-1a and Bu-1b. However, AV20 identified B cells in partially inbred birds as well as inbred lines including line 6 1 and line 7 2, indicating that it recognises a monomorphic determinant. AV20 immunoprecipitated an antigen with a Mw r of 150 kDa under non-reducing conditions and 70–75 kDa under reducing conditions indicating it is a homodimer. Serial immunoprecipitations of bursal-cell lysates from line 6 1 or line 7 2 confirmed that AV20 recognised the same antigen as mAbs against Bu-1a and Bu-1b in the respective lines.