Dog-DAT: a direct agglutination test using stabilized, freeze-dried antigen for the serodiagnosis of canine visceral leishmaniasis

We have evaluated the use of an improved direct agglutination test (DAT) based on stable, freeze-dried antigen for the detection of anti-Leishmania antibodies in canine serum samples. With a cut-off value of 1:640, the sensitivity of the DAT was shown to be 100% and the specificity of the test was 9...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 1996-12, Vol.16 (3/4), p.235-239
Main Authors: Oskam, L, Slappendel, R.J, Beijer, E.G.M, Kroon, N.C.M, Ingen, C.W. van, Ozensoy, S, Ozbel, Y, Terpstra, W.J
Format: Article
Language:English
Subjects:
Agglutination
Agglutination Tests - methods
animal health
animal parasites and pests
Animal protozoal diseases
Animals
Antibodies
Antibodies, Protozoan - blood
Antigens
Antigens, Protozoan
Biological and medical sciences
Canine visceral leishmaniasis
Direct agglutination test
Dog Diseases - diagnosis
Dogs
Evaluation Studies as Topic
Freeze Drying
Infectious diseases
Leishmania
Leishmaniasis, Visceral - diagnosis
Leishmaniasis, Visceral - epidemiology
Leishmaniasis, Visceral - veterinary
Medical sciences
Netherlands - epidemiology
Parasitic diseases
Protozoa
Protozoal diseases
Sensitivity and Specificity
Turkey - epidemiology
Vector-borne diseases
Visceral leishmaniasis
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Summary:We have evaluated the use of an improved direct agglutination test (DAT) based on stable, freeze-dried antigen for the detection of anti-Leishmania antibodies in canine serum samples. With a cut-off value of 1:640, the sensitivity of the DAT was shown to be 100% and the specificity of the test was 98.8%.
ISSN:0928-8244
1574-695X
2049-632X
DOI:10.1111/j.1574-695X.1996.tb00141.x