Expression of genes for bone morphogenetic proteins and receptors in human dental pulp

Bone morphogenetic proteins (BMP) have been shown to induce reparative dentine formation experimentally but the cells responsible, which respond to BMPs, have not been identified. The BMP signal is probably mediated by interaction of type I and II BMP receptors (R). Here, the RNA of human adult dent...

Full description

Saved in:
Bibliographic Details
Published in:Archives of oral biology 1996-10, Vol.41 (10), p.919-923
Main Authors: Gu, Keni, Smoke, Richard H., Rutherford, R.Bruce
Format: Article
Language:English
Subjects:
Activin Receptors, Type I
Adult
Base Sequence
bone morphogenetic protein
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 4
Bone Morphogenetic Protein 7
bone morphogenetic protein receptor types I and II
Bone Morphogenetic Proteins - biosynthesis
Bone Morphogenetic Proteins - genetics
Chromosome Mapping
dental pulp
Dental Pulp - chemistry
Dental Pulp - metabolism
Dentin, Secondary - growth & development
Dentinogenesis - genetics
Dentistry
Gene Expression
Humans
In Situ Hybridization
Molecular Sequence Data
Polymerase Chain Reaction
Receptors, Growth Factor - biosynthesis
Receptors, Growth Factor - genetics
RNA, Messenger - analysis
Transforming Growth Factor beta
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bone morphogenetic proteins (BMP) have been shown to induce reparative dentine formation experimentally but the cells responsible, which respond to BMPs, have not been identified. The BMP signal is probably mediated by interaction of type I and II BMP receptors (R). Here, the RNA of human adult dental pulp and pulp cells in culture was examined by reverse transcription (RT) polymerase chain reaction (PCR) for evidence of mRNA for BMPs. mRNAs for BMP-2, -4, osteogenic protein-1, ActR-l (activin-like kinase receptor), BMPR-IA, -IB and -II were detected by RT-PCR. The 698-bp PCR fragment for BMPR-IB was used to probe pulp cells for expression of that receptor. Cell expression of BMPR-IB was detected by the hybridization probe. The findings suggest that resident pulp cells may be able to respond to BMPs to initiate tissue formation.
ISSN:0003-9969
1879-1506
DOI:10.1016/S0003-9969(96)00052-0