Serological studies of monoclonal RH1(D) antibodies with RH1(D) variants

In this paper we chose to emphasize three aspects of our work. First we underlined that “low grade and high grade” D weak red blood cells studied at the DNA level could, when monoclonal antibodies were used, give patterns of positive and negative reactions like partial RH1(D) cells. Secondly, we sho...

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Bibliographic Details
Published in:Transfusion clinique et biologique (Paris) 1996, Vol.3 (6), p.401-404
Main Authors: Le Pennec, P.-Y., Noizat-Pirenne, F., Klein, M.T., Fraval, D., Lascaux, J.M., Rouger, Ph
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Biological and medical sciences
Hematology
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Monoclonal RH1(d) antibodies
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Rh-Hr Blood-Group System - immunology
serological studies
Serology
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Summary:In this paper we chose to emphasize three aspects of our work. First we underlined that “low grade and high grade” D weak red blood cells studied at the DNA level could, when monoclonal antibodies were used, give patterns of positive and negative reactions like partial RH1(D) cells. Secondly, we showed the importance of the technical conditions of the study which are essential for establishing a pattern of reactivity defining an epitope. It appears that the use of papain treated cells at room temperature can be misleading for the definition of epitope especially with IgM antibodies. Lastly we pointed out the interest of Rh variant cells, defined at the gene level, to study the expression of RH1(D) epitopes on the external part of the membrane.
ISSN:1246-7820
DOI:10.1016/S1246-7820(96)80053-X