Post-castration rebound of an androgen regulated prostatic gene

After castration, the rat dorsolateral prostate M-40 mRNA initially decreased then rebounded to precastrated levels. The cellular site of M-40 expression and its renewed expression after castration was defined by in situ hybridization histochemistry. In situ hybridization with either a 32P-labeled o...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 1988-11, Vol.84 (1), p.3-15
Main Authors: Sweetland, R, Sheppard, P C, Dodd, J G, Matusik, R J
Format: Article
Language:English
Subjects:
Androgens - physiology
Animals
DNA Probes
Genes
Male
Nucleic Acid Hybridization
Orchiectomy
Organ Size
Prostate - anatomy & histology
Prostate - physiology
Rats
Rats, Inbred Strains
RNA - genetics
RNA - isolation & purification
RNA, Messenger
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Summary:After castration, the rat dorsolateral prostate M-40 mRNA initially decreased then rebounded to precastrated levels. The cellular site of M-40 expression and its renewed expression after castration was defined by in situ hybridization histochemistry. In situ hybridization with either a 32P-labeled or biotin-labeled M-40 cDNA probe demonstrated that M-40 mRNA levels were higher in the lateral than dorsal prostate. A second androgen regulated gene, RWB, also was highly expressed in the lateral prostate. The biotinylated cDNA probes provided microscopic resolution of the expressing cells, revealing two distinct morphologies of lateral epithelium which expressed both the M-40 and RWB mRNA. These morphologies appeared in ducts which contained either epithelial cell sheets that were highly convoluted or thinner epithelial cells with a minimal degree of convolution. The RWB mRNA decreased in both cell populations in response to androgen withdrawal. The decline and reappearance of M-40 mRNA also appeared in both epithelial cell types. These data demonstrated that after castration the M-40 mRNA initially decreased as expected for an androgen sensitive gene and then progressed to a fully inducible state. The mechanism of this progression remains to be elucidated.
ISSN:0300-8177
1573-4919
DOI:10.1007/BF00235188