Fc gamma-receptor III (CD 16) is involved in NK-B cell interaction

CD16, the low affinity receptor for monomeric IgG (Fc gamma RIIIA), is a well characterized activation molecule on NK cells. In this study we investigated the role of CD16 in NK cell-mediated regulation of immunoglobulin production. Cocultures of the CD16+ human NK clone CNK6 and highly purified SAC...

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Bibliographic Details
Published in:Immunobiology (1979) 1996, Vol.196 (4), p.387-398
Main Authors: Lenz, P, Gessner, J E, Sautes, C, Schmidt, R E
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - pharmacology
B-Lymphocytes - immunology
B-Lymphocytes - metabolism
Cell Communication - immunology
Coculture Techniques
Cytokines - biosynthesis
Cytokines - genetics
Humans
Immunoglobulins - biosynthesis
Killer Cells, Natural - immunology
Killer Cells, Natural - metabolism
Lymphocyte Activation
Protein Binding - immunology
Receptors, IgG - immunology
Receptors, IgG - metabolism
Receptors, IgG - physiology
Solubility
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Summary:CD16, the low affinity receptor for monomeric IgG (Fc gamma RIIIA), is a well characterized activation molecule on NK cells. In this study we investigated the role of CD16 in NK cell-mediated regulation of immunoglobulin production. Cocultures of the CD16+ human NK clone CNK6 and highly purified SAC/IL-2-activated B lymphocytes with various CD16 antibodies showed significantly diminished NK-enhanced immunoglobulin production in a dose-dependent manner, indicating that CD16 is relevant in NK-B cell interaction. Similarly, recombinant soluble CD16 incubated with B cells before cultures, suppressed the NK cell-stimulated B cell antibody response. Enhanced immunoglobulin production was also inhibited by Fc-specific F(ab')2 anti-body fragments. Coculture of NK cells with B lymphocytes resulted in induction of mRNA for IFN-gamma and TNF-alpha. The accumulation of mRNA for these cytokines was prevented by addition of CD16 and Fc-specific antibodies. It is proposed that interaction of CD16 on NK cells with B cell bound immunoglobulin leads to induction of cytokines in NK cells which stimulate immunoglobulin production by B cells.
ISSN:0171-2985
DOI:10.1016/S0171-2985(96)80061-1