Tyrosine phosphorylation of measles virus P-phosphoprotein in persistently infected neuroblastoma cells

Replication and encapsidation of measles virus (MV) requires the interaction between the nuclear protein (N) and the phosphoprotein (P). It is known that both proteins are phosphorylated on serine and threonine residues. Recently we have shown that N is phosphorylated on tyrosine in persistently-inf...

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Bibliographic Details
Published in:Virus genes 1996, Vol.13 (3), p.203-210
Main Authors: Ofir, R, Weinstein, Y, Bazarsky, E, Blagerman, S, Wolfson, M, Hunter, T, Rager-Zisman, B
Format: Article
Language:English
Subjects:
Animals
measles virus
Measles virus - metabolism
Mice
Neuroblastoma
Phosphoproteins - genetics
Phosphoproteins - metabolism
Phosphorylation
Protein Binding
Protein-Tyrosine Kinases - metabolism
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Serine - metabolism
src-Family Kinases
Threonine - metabolism
Tumor Cells, Cultured
Tyrosine - metabolism
Viral Proteins - genetics
Viral Proteins - metabolism
Virus Latency
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Summary:Replication and encapsidation of measles virus (MV) requires the interaction between the nuclear protein (N) and the phosphoprotein (P). It is known that both proteins are phosphorylated on serine and threonine residues. Recently we have shown that N is phosphorylated on tyrosine in persistently-infected mouse neuroblastoma cells (NS20Y/MS). Here, we show that P in NS20Y/MS is also phosphorylated on tyrosine. To investigate whether cellular tyrosine kinases can bind and phosphorylate P, a solid phase kinase assay was employed. We show that bacterially-expressed MV P fragments, were phosphorylated on tyrosine by purified mouse c-Src protein-tyrosine kinase and when mixed with uninfected neuroblastoma cell (NS20Y) extracts, these P fragments were phosphorylated on tyrosine in addition to serine and threonine. These results imply that MV P is a substrate for tyrosine phosphorylation by cellular tyrosine kinase(s).
ISSN:0920-8569
1572-994X
DOI:10.1007/BF00366980