Decreased prereceptorial glucocorticoid activating capacity in starvation due to an oxidative shift of pyridine nucleotides in the endoplasmic reticulum

Redox state of pyridine nucleotides of the endoplasmic reticulum (ER) lumen was determined in different nutritional conditions. NADPH-dependent cortisone reduction and NADP +-dependent cortisol oxidation were measured in rat liver microsomes, by utilizing the luminal 11β-hydroxysteroid dehydrogenase...

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Bibliographic Details
Published in:FEBS letters 2010-11, Vol.584 (22), p.4703-4708
Main Authors: Kereszturi, Éva, Kálmán, Fanni S., Kardon, Tamás, Csala, Miklós, Bánhegyi, Gábor
Format: Article
Language:English
Subjects:
11-beta-Hydroxysteroid Dehydrogenase Type 1 - metabolism
11β-Hydroxysteroid dehydrogenase type 1
11βHSD1
Animals
Cortisone - metabolism
Endoplasmic reticulum
Endoplasmic Reticulum - metabolism
G6P
G6PT
Glucocorticoid
glucose-6-phosphate
glucose-6-phosphate transporter
H6PD
hexose-6-phosphate dehydrogenase
Hydrocortisone - metabolism
Liver - cytology
Liver - pathology
Male
Microsomes, Liver - metabolism
NADP - metabolism
NADPH
Oxidation-Reduction
Rats
Rats, Wistar
Redox
Starvation - metabolism
Starvation - pathology
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Summary:Redox state of pyridine nucleotides of the endoplasmic reticulum (ER) lumen was determined in different nutritional conditions. NADPH-dependent cortisone reduction and NADP +-dependent cortisol oxidation were measured in rat liver microsomes, by utilizing the luminal 11β-hydroxysteroid dehydrogenase type 1 activity. Cortisone reduction decreased, while cortisol oxidation increased during onward starvation, showing that the luminal NADPH/NADP + ratio was substantially decreased. Cortisone or metyrapone addition caused a smaller decrease in NADPH fluorescence in microsomes from starved rats. The results demonstrate that nutrient supply is mirrored by the redox state of ER luminal pyridine nucleotides.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2010.10.053