A BALB/c mouse model shows that liver involvement in dengue disease is immune-mediated

In the present study, BALB/c mice were used to develop a model for the hepatic injury associated to dengue infection. Histological analysis after subcutaneous inoculation with a low viral dose of dengue-2 virus showed Kupffer cell hyperplasia and an increased inflammatory cellular infiltrate next to...

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Bibliographic Details
Published in:Experimental and molecular pathology 2010-12, Vol.89 (3), p.321-326
Main Authors: de Oliveira França, Rafael Freitas, Zucoloto, Sérgio, Antônio Lopes da Fonseca, Benedito
Format: Article
Language:English
Subjects:
Animal model
Animals
Arboviroses
Biological and medical sciences
Cytokine
Cytokines - biosynthesis
Dengue
Dengue - complications
Dengue - immunology
Dengue - pathology
Dengue fevers
Disease Models, Animal
Female
Gene Expression
Human viral diseases
Infectious diseases
Inflammation
Investigative techniques, diagnostic techniques (general aspects)
Liver
Liver Diseases - immunology
Liver Diseases - pathology
Liver Diseases - virology
Medical sciences
Mice
Mice, Inbred BALB C
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
RNA, Messenger - analysis
RNA, Viral - analysis
Transcription, Genetic
Tropical viral diseases
Viral diseases
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Summary:In the present study, BALB/c mice were used to develop a model for the hepatic injury associated to dengue infection. Histological analysis after subcutaneous inoculation with a low viral dose of dengue-2 virus showed Kupffer cell hyperplasia and an increased inflammatory cellular infiltrate next to the bile ducts on days 5, 7 and 14 post-inoculation, mainly characterized by the presence of mononuclear cells. The liver mRNA transcription level of IL-1β was highest on the 5th day post-infection ( p.i.) and decreased by the 21st day, TNF-α showed a peak of mRNA transcription after 14 days p.i. coinciding with the regression of cellular infiltrates and elevated expression of TGF-β mRNA. Serum AST and ALT levels were slightly elevated at 7 and 14 days post-infection. Dengue-2 RNA levels were undetectable in the liver on any of the days following inoculation. Our observations suggest that, as it is true for humans, the animals undergo a transient and slight liver inflammation, probably due to local cytokine production and cellular infiltration in the liver.
ISSN:0014-4800
1096-0945
DOI:10.1016/j.yexmp.2010.07.007