Spermatozoa Bound to Solid State Hyaluronic Acid Show Chromatin Structure With High DNA Chain Integrity: An Acridine Orange Fluorescence Study

During human spermiogenesis, the elongated spermatids undergo a plasma membrane remodeling step that facilitates formation of the zona pellucida and hyaluronic acid (HA) binding sites. Various biochemical sperm markers indicated that human sperm bound to HA exhibit attributes similar to that of zona...

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Bibliographic Details
Published in:Journal of andrology 2010-11, Vol.31 (6), p.566-572
Main Authors: Yagci, Artay, Murk, William, Stronk, Jill, Huszar, Gabor
Format: Article
Language:English
Subjects:
Acridine Orange - chemistry
Biological and medical sciences
Chromatin - chemistry
DNA - chemistry
DNA Fragmentation
Fluorescent Dyes - chemistry
Fundamental and applied biological sciences. Psychology
Gynecology. Andrology. Obstetrics
Humans
Hyaluronic Acid - chemistry
Male
Male genital diseases
Mammalian male genital system
Medical sciences
Sperm Injections, Intracytoplasmic
Sperm maturity
sperm selection
Spermatozoa - chemistry
Vertebrates: reproduction
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Summary:During human spermiogenesis, the elongated spermatids undergo a plasma membrane remodeling step that facilitates formation of the zona pellucida and hyaluronic acid (HA) binding sites. Various biochemical sperm markers indicated that human sperm bound to HA exhibit attributes similar to that of zona pellucida–bound sperm, including minimal DNA fragmentation, normal shape, and low frequency of chromosomal aneuploidies. In this work, we tested the hypothesis that HA‐bound sperm would be enhanced in sperm of high DNA chain integrity and green acridine orange fluorescence (AOF) compared with the original sperm in semen. Sperm DNA integrity in semen and in their respective HA‐bound sperm fractions was studied in 50 men tested for fertility. In the semen samples, the proportions of sperm with green AOF (high DNA integrity) and red AOF (DNA breaks) were 54.9% ± 2.0% and 45.0% ± 1.9%, whereas in the HA‐bound sperm fraction, the respective proportions were 99% and 1.0%, respectively. The data indeed demonstrated that HA shows a high degree of selectivity for sperm with high DNA integrity. These findings are important from the points of view of human sperm DNA integrity, sperm function, and the potential efficacy of HA‐mediated sperm selection for intracytoplasmic sperm injection.
ISSN:0196-3635
1939-4640
DOI:10.2164/jandrol.109.008912