Chemically modified MEMS probe driven by self-alignment mechanism for photooxidative cell membrane perforation

This paper describes a photooxidative cell membrane perforator that can improve intracellular manipulations. The cell membrane perforator was developed as a MEMS interface employing an ultraviolet excitation of a photosensitizer. The perforator consists of a movable planar probe and a suction hole h...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2010-06, Vol.148 (1), p.29-33
Main Authors: Tonomura, Wataru, Yamamoto, Takahiro, Saito, Takashi K., Konishi, Satoshi
Format: Article
Language:English
Subjects:
Actuators
Bonding
Chemically modified probe
Excitation
Gold
Intracellular manipulation
Membranes
Microelectromechanical systems
Micrometers
Perforation
Photooxidative cell membrane perforation
Photosensitizer
Self-alignment mechanism
Ultraviolet
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Summary:This paper describes a photooxidative cell membrane perforator that can improve intracellular manipulations. The cell membrane perforator was developed as a MEMS interface employing an ultraviolet excitation of a photosensitizer. The perforator consists of a movable planar probe and a suction hole having a self-alignment mechanism to aid manipulation. The tip of the probe modified with α-terthienyl is introduced to the cell clamped at the pre-aligned suction hole (Φ 5μm in diameter). The α-terthienyl is immobilized using the Au–S bond at the tip of the probe, which is 5μm wide and 400nm thick. The cell membrane is perforated by ultraviolet excitation of the α-terthienyl as a photosensitizer. Photooxidative perforation of the cell membrane was successfully performed using PC12 cells with a diameter as small as tens of micrometers.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2010.04.014