Software-based quantitation of bioassays on CD

Compact discs (CDs) can be used as rapid, low-cost, high-capacity screening platforms for running bioassays with no modification of the detection hardware (conventional standard optical drive). We describe herein a new protocol to read and quantitate biotin–streptavidin binding assays with a standar...

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Published in:Sensors and actuators. B, Chemical Chemical, 2010-07, Vol.148 (2), p.620-623
Main Authors: Pallapa, Manu, Ou, Lily M.L., Parameswaran, M. (Ash), Yu, Hua-Zhong
Format: Article
Language:English
Subjects:
Binding
Bioassay
Biomarker detection
Cadmium
Compact disks
Computer programs
Error detection
Format
IsoBuster
Software
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description Compact discs (CDs) can be used as rapid, low-cost, high-capacity screening platforms for running bioassays with no modification of the detection hardware (conventional standard optical drive). We describe herein a new protocol to read and quantitate biotin–streptavidin binding assays with a standard optical drive by using a CD-data analysis software (IsoBuster), which identifies erroneous sectors by locating the exact error position bit-by-bit and allows various data formats to be used. The numbers of erroneous sectors increase as a function of the streptavidin concentration in the tested solutions. High spatial accuracy and detection sensitivity (0.3 μg/mL) were achieved.
doi_str_mv 10.1016/j.snb.2010.05.045
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subjects Binding
Bioassay
Biomarker detection
Cadmium
Compact disks
Computer programs
Error detection
Format
IsoBuster
Software
title Software-based quantitation of bioassays on CD
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