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Detection of single lipid bilayers with coherent anti-Stokes Raman scattering (CARS) microscopy

We investigated vibrational imaging of phospholipid bilayers with CARS microscopy. Single lipid membranes of supported bilayers, giant unilamellar vesicles and intact erythrocyte membrane are detected with the strong resonant signal of the C–H stretching vibration. In addition, it is shown that the...

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Bibliographic Details
Published in:Journal of Raman spectroscopy 2003-09, Vol.34 (9), p.642-650
Main Authors: Potma, Eric O., Xie, X. S.
Format: Article
Language:English
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Summary:We investigated vibrational imaging of phospholipid bilayers with CARS microscopy. Single lipid membranes of supported bilayers, giant unilamellar vesicles and intact erythrocyte membrane are detected with the strong resonant signal of the C–H stretching vibration. In addition, it is shown that the CARS signal field of the lipids near the glass/water interface is amplified through mixing with the back‐reflected non‐resonant CARS field of the glass coverslip. Furthermore, interference effects between two separate bilayers are observed, allowing intermembrane distances to be determined beyond the diffraction‐limited resolution of the microscope. Copyright © 2003 John Wiley & Sons, Ltd.
ISSN:0377-0486
1097-4555
DOI:10.1002/jrs.1045