Biochemical properties of canine serum ferritin: iron content and nonbinding to concanavalin A

A sandwich enzyme-linked immunosorbent assay using H-subunit-rich canine heart ferritin as a standard has been developed for measuring canine serum ferritin which is H-subunit-rich. Serum ferritin concentrations in 51 normal dogs ranged from 143 to 1766 ng ml(-1), with a mean value of 479 +/- 286 (S...

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Bibliographic Details
Published in:Biometals 2000-12, Vol.13 (4), p.319-324
Main Authors: Watanabe, K, Muranishi, N, Murata, Y, Orino, K, Okano, S, Yamamoto, S
Format: Article
Language:English
Subjects:
Anemia
Animals
Biochemistry
Concanavalin A
Concanavalin A - metabolism
Dogs
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Ferritin
Ferritins - blood
Ferritins - chemistry
Ferritins - immunology
Heat treatment
Immunochemistry
In Vitro Techniques
Iron
Iron - analysis
Iron - blood
Liver - chemistry
Myocardium - chemistry
Protein Binding
Protein Subunits
Proteins
Reference Standards
Serums
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Summary:A sandwich enzyme-linked immunosorbent assay using H-subunit-rich canine heart ferritin as a standard has been developed for measuring canine serum ferritin which is H-subunit-rich. Serum ferritin concentrations in 51 normal dogs ranged from 143 to 1766 ng ml(-1), with a mean value of 479 +/- 286 (SD) ng ml(-1). Serum ferritin iron concentrations as determined by an immunoprecipitation technique ranged from 30.4 to 115.9 ng ml(-1) in 15 normal dogs with serum ferritin protein levels of 298 to 959 ng ml(-1). There was a significant linear correlation between the serum ferritin iron and protein levels (r=0.9441, P
ISSN:0966-0844
1572-8773
DOI:10.1023/A:1009276323707