Influence of culture conditions on thermostable lipase production by a thermophilic alkalitolerant strain of Bacillus sp

The effect of different culture conditions on thermostable lipase production by Bacillus sp. was studied in shake flasks. A maximum enzyme activity of 67-75 nkat/mL was observed in a medium consisting of 0.5% soybean flour and 0.1% stearyl glycerol esters or natural fats. A lipase activity of about...

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Bibliographic Details
Published in:Folia microbiologica 1996, Vol.41 (2), p.146-148
Main Authors: KAMBOUROVA, M, EMANUILOVA, E, DIMITROV, P
Format: Article
Language:English
Subjects:
Bacillus - growth & development
Bacillus - metabolism
Bacteriological Techniques
Biological and medical sciences
Bioreactors
Biotechnology
Culture Media
Enzyme engineering
Esters - metabolism
Fats - metabolism
Fermentation
Fundamental and applied biological sciences. Psychology
Glycine max - metabolism
Lipase - biosynthesis
Methods. Procedures. Technologies
Production of selected enzymes
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Summary:The effect of different culture conditions on thermostable lipase production by Bacillus sp. was studied in shake flasks. A maximum enzyme activity of 67-75 nkat/mL was observed in a medium consisting of 0.5% soybean flour and 0.1% stearyl glycerol esters or natural fats. A lipase activity of about 117 nkat/mL was established when the cultivation was carried out in a laboratory fermentor at 20% minimal dissolved oxygen level, the enzyme production being increased 1.5 fold compared to that in a flask culture.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02814690