A proton magnetic resonance spectroscopy study of aging and transformed human fibroblasts

Proton magnetic resonance spectroscopy ( 1H MRS) has been used to monitor changes occurring during aging and transformation in human lung fibroblasts. Aging was studied in MRC-5 cells from nonsenescent (early passage) to presenescent (late passage) and senescence. Nonsenescent cells infected with SV...

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Bibliographic Details
Published in:Experimental gerontology 1996-11, Vol.31 (6), p.669-686
Main Authors: Rutter, Allison, MacKinnon, Wanda B., Huschtscha, Lily I., Mountford, Carolyn E.
Format: Article
Language:English
Subjects:
aging
Cell Line, Transformed
Cellular Senescence
Cholesterol - analysis
Choline - analysis
Fibroblasts - physiology
human lung fibroblasts
Humans
Magnetic Resonance Spectroscopy
Membrane Lipids - analysis
Simian virus 40
transformation
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Summary:Proton magnetic resonance spectroscopy ( 1H MRS) has been used to monitor changes occurring during aging and transformation in human lung fibroblasts. Aging was studied in MRC-5 cells from nonsenescent (early passage) to presenescent (late passage) and senescence. Nonsenescent cells infected with SV40 virus (pretransformed) were monitored through crisis and subsequent immortalization. Aging changes were observed with one- and two-dimensional MR spectra. Cholesterol and lipid resonances were significantly increased from nonsenescent cultures to senescence. These changes could be caused by chemical or structural changes in the plasma membrane or in intracellular lipid pools. In contrast, choline levels rose from nonsenescent to presenescent cells but at senescence dropped to that of nonsenescent cells. Increased choline levels are often associated with increased cellular proliferation. After SV40 infection of MRC-5 cells there was an increase of cholesterol and lipid levels that peaked at crisis. Newly immortalized cells exhibited a drop in cholesterol and lipid to nonsenescent cell levels, but these rose again in established immortalized cells. In contrast to presensescent cultures, the levels of choline gradually increased from pretransformed to crisis phase but still continued to rise after immortalization. Thus, 1H MRS illustrates similarities in lipid behavior at senescence and crisis, whereas the choline levels are different.
ISSN:0531-5565
1873-6815
DOI:10.1016/S0531-5565(96)00076-9