A Simple Freeze-Drying Device Using t-Butyl Alcohol for SEM Specimens

In a simple newly developed freeze drying device, the biological specimens immersed and frozen in t-butanol were evacuated in a vacuum chamber by an oil rotary pump to dry the tissues. When a metal block precooled by liquid nitrogen was placed in the chamber, the cold metal block adsorbed the sublim...

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Bibliographic Details
Published in:Journal of electron microscopy 1988, Vol.37 (6), p.351-352
Main Authors: AKAHORI, Hiroshi, ISHII, Hiroko, NONAKA, Ikuya, YOSHIDA, Hisaharu
Format: Article
Language:English
Subjects:
1-Butanol
Animals
Butanols
cold metal block
Epithelium - ultrastructure
Freeze Drying - instrumentation
freeze-dry
Mice
Microscopy, Electron, Scanning
oil rotary pump
t-butanol
Trachea - ultrastructure
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Description
Summary:In a simple newly developed freeze drying device, the biological specimens immersed and frozen in t-butanol were evacuated in a vacuum chamber by an oil rotary pump to dry the tissues. When a metal block precooled by liquid nitrogen was placed in the chamber, the cold metal block adsorbed the sublimated t-butanol and prevented its contamination into the oil of the rotary pump.
ISSN:0022-0744
1477-9986
1477-9986
DOI:10.1093/oxfordjournals.jmicro.a050704