Cellular heterogeneity in cathepsin D distribution in equine articular cartilage

Summary The distribution of cathepsin D in normal equine growth cartilage has been examined immunocytochemically using an antiserum raised against human cathepsin D. The cross‐reactivity and specificity of the antiserum for equine cathepsin D was confirmed, and its lysosomal localisation was demonst...

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Bibliographic Details
Published in:Equine veterinary journal 1997-07, Vol.29 (4), p.267-273
Main Authors: HERNANDEZ-VIDAL, G., JEFFCOTT, L. B., DAVIES, M. E.
Format: Article
Language:English
Subjects:
Animals
Antibody Specificity
articular cartilage
Cartilage, Articular - cytology
Cartilage, Articular - enzymology
Cartilage, Articular - pathology
cathepsin D
Cathepsin D - analysis
Cathepsin D - immunology
chondrocytes
Chondrocytes - enzymology
Cross Reactions
horse
Horses - metabolism
Immune Sera - immunology
Immunohistochemistry - veterinary
Microscopy, Confocal - veterinary
Sheep
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Summary:Summary The distribution of cathepsin D in normal equine growth cartilage has been examined immunocytochemically using an antiserum raised against human cathepsin D. The cross‐reactivity and specificity of the antiserum for equine cathepsin D was confirmed, and its lysosomal localisation was demonstrated in horse skin fibroblasts by confocal scanning microscopy. Cultured horse chondrocytes were heterogenous in their expression of cathepsin D. Heterogeneity of distribution of the enzyme was also seen in chondrocytes in cartilage from different anatomical sites. A high level of cathepsin D was observed in the deep layer of cartilage from the lateral trochlear ridge of the distal femur. Cathepsin D was absent in the hypertrophic zone of the distal radial growth plate.
ISSN:0425-1644
2042-3306
DOI:10.1111/j.2042-3306.1997.tb03122.x